نتایج جستجو برای: phosphate gradient method
تعداد نتایج: 1815145 فیلتر نتایج به سال:
A highly sensitive analytical method was developed that allows the separation of ganglioside mixtures and quantification of individual non-derivatized gangliosides in the concentration range between 2 pmol and 1 nmol. Gangliosides were separated with a gradient of acetonitrile/phosphate buffer on a 1 mm diameter microbore HPLC column packed with Spherisorb-NH2. They eluted according to their nu...
Sharon Bio-Medicine Ltd, Plot L6, Taloja-MIDC, TalukaPanvel, Dist Raigad410 208, Maharashtra, India Shri Jagdishprasad Jhabarmal Tibrewala University, Vidya Nagari, Churu-Bishau Road, Chudela, Dist. Jhunjhunu333 001, Rajasthan, India ABSTRACT A simple, precise and stability-indicating HPLC method was developed and validated for the simultaneous determination of anti-hypertensive drugs Irbesarta...
The main objective of the work discussed in this paper was evaluation of a chromatographic method for simultaneous determination of hydrochlorothiazide (HCTZ), lisinopril (L), and their impurities in pharmaceuticals. Chlorothiazide (CTZ) and disulfonamide (DSA), as potential impurities in hydrochlorothiazide, and diketopiperazine (DKP), as an impurity of lisinopril, were analyzed. The chromatog...
A novel approach was used to develop and validate a rapid, specific, accurate and precise reverse phase ultra performance liquid chromatographic (UPLC) method for the simultaneous determination of Sitagliptin phosphate monohydrate and Metformin hydrochloride in pharmaceutical dosage forms. The chromatographic separation was achieved on Aquity UPLC BEH C8 100 × 2.1 mm, 1.7 μm, column using a buf...
With respect to importance of the conjugate gradient methods for large-scale optimization, in this study a descent three-term conjugate gradient method is proposed based on an extended modified secant condition. In the proposed method, objective function values are used in addition to the gradient information. Also, it is established that the method is globally convergent without convexity assu...
In this paper, an efficient conjugate gradient method for unconstrained optimization is introduced. Parameters of the method are obtained by solving an optimization problem, and using a variant of the modified secant condition. The new conjugate gradient parameter benefits from function information as well as gradient information in each iteration. The proposed method has global convergence und...
objective: the aim of this study was to examine the efficiency of ha-binding test and sperm gradient processing method for the selection of sperm with normal protamine, intact dna and normal morphology. materials and methods: the study was conducted on 29 patients undergoing icsi. semen analysis was carried out according to who criteria. semen samples were divided into three equal portions. one...
A new learning strategy is proposed for training of radial basis functions (RBF) network. We apply two different local optimization methods to update the output weights in training process, the gradient method and a combination of the gradient and Newton methods. Numerical results obtained in solving nonlinear integral equations show the excellent performance of the combined gradient method in ...
THE PEPTIC ZYMOGENS OF HUMAN GASTRIC MUCOSA HAVE BEEN STUDIED IN THE PAST BY THREE DIFFERENT TECHNIQUES: phosphate-gradient chromatography on a column of DEAE-cellulose, chloride-gradient chromatography on DEAE-cellulose and agar-gel electrophoresis. These techniques have given different results, each suggesting the presence of a different number of pepsin zymogens in human gastric mucosa. In t...
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