microscopy as “field based test” was compared with is900 blood pcr for the diagnosis and estimationof prevalence of mycobacterium avium subspecies paratuberculosis (map) in domestic livestock withclinical johne’s disease (jd). of 252 animals screened, 39.3 and 13.1% were positive by faecal microscopyand blood pcr, respectively. proportional agreement between “microscopy” and blood pcr was subst...

purpose: to image leukocyte adherence to the vascular endothelium and extravasation into surrounding tissues in neovascularized corneas using acridine orange, a selective dna stain, in rats methods: corneal neovascularization was induced by placing two sutures in the cornea of one eye of 12 long-evans rats. one week later, acridine orange (4 mg/kg) was injected via the tail vein and the eyes we...

introduction: skeletal staining is an important part of anatomical studies which can evaluate macroscopic disorders in bones and cartilages development. the aim of the present article is to illustrate two distinct protocols by all details in order to stain skeletal system of the lab animals in all ages of their lives so it becomes easy for future studies in this field. methods: in this paper, s...

introduction: unstained formalin-fixed whole brain specimens and brain slices do not give satisfactory results for teaching neuroanatomy. in addition, difficulties in obtaining human brains for dissection have increased the demand for more durable brain specimens that have been obtained by the plastination technique. in the present study brain specimens were sliced, fixed and stained using the ...

statement of problem: due to the growing popularity of vital bleaching by carbamide peroxide it is imperative to understand the effect of such agents on enamel and dentine. purpose: the purpose of this study was to evaluate the effect of a 16% carbamide peroxide bleaching gel; vivastyle on enamel staining susceptibility. materials and methods: thirty bovine specimens were selected and randomly ...

abstract: introduction & objective: the hippocampal formation is a part of limbic system that plays an important role in memory and learning. the hippocampus is divided into three subfields: ca1, ca2 and ca3. in addition to pyramidal neurons and interneurons, the astrocytes play important roles in hippocampus, probably in memory and learning. in fact, learning needs some instruments for informa...

background: oocyte maturation and subsequent in vitro production (ivp) of embryos are affected by diverse groups of chemicals in maturation medium which are needed for successful mammalian oocyte maturation during which the dramatic cytoplasmic and nuclear reprogramming events take place. this study was designed to evaluate the effects of protein source (fetal bovine serum, fbs, and bovine seru...

background : this study aimed to compare three staining methods including: calcofluor white, chromotrope and quick hot gram chromotrope used in diagnosis of intestinal microsporidial spores . methods : one hundred and seventy five stool specimens were collected from patients referred to laboratory of intestinal protozoology at the school of public health, tehran university of medical sciences d...