Plant growth and activity of peroxidases (POX) and phenylalanine ammonia-lyase (PAL) were investigated in seedlings of yellow lupine (Lupinus luteus L.) and soybean (Glycine max L.) treated with copper (25 mg/l) and an inhibitor of ethylene biosynthesis (CoCl 2 ). Both lupine and soybean plants stressed with an excess of copper exhibited a decrease in root length and fresh weight. In lupine pla...

Phenylalanine ammonia-lyase, which irreversibly deaminates phenylalanine and tyrosine, markedly inhibited the growth of human leukemic and murine L5178Y lymphoblasts in vitro but had a negligible effect on resting (nondividing) normal lymphocytes. These studies also demonstrated that phenylalanine ammonia-lyase inhibited DNA synthesis in leukemic cells at least as effectively as did asparaginas...

Glyphosate showed a remarkable effect inducing the change of flower symmetry from the actinomorphic to the zygomorphic type in Petunia hybrida. Glyphosate [N-(phosphonomethyl)glycine] reduced the anthocyanin content and showed a weak inhibitory effect against phenylalanine ammonia-lyase (PAL) activity. L-2-Aminooxy-3-phenylpropionic acid (APA), an inhibitor of PAL activity, reduced the anthocya...

The transcript levels of six defense genes including pathogenesis-related gene 1 (PR-1), pathogenesis-related gene 2 (PR-2), pathogenesis-related gene 5 (PR-5), lipoxygenase (LOX), phenylalanine ammonia-lyase (PAL) and catalase (CAT) were investigated in tomato plants inoculated with Xanthomonas axonopodis pv. phaseoli as a non-host pathogen and X. euvesicatoria as a host pathogen. Activation o...

Freshly harvested tomato fruit (Solanum lycopersicum) were pre-treated with 0, 50, and 100 µM prohexadione-calcium (Pro-Ca) and then stored at 1 °C for 21 days to investigate the effect of Pro-Ca treatment on electrolyte leakage (EL), malondialdehyde (MDA), proline and total phenols contents, and activity of phenylalanine ammonia-lyase (PAL) in relation to chilling injury (CI).Treatment with Pr...

This study was conducted to investigate certain characteristics of the purified Phenylalanine ammonia lyase (PAL). The results revealed that molecular weight 180 kDa by gel filtration and Native-PAGE. optimal pH temperature for enzyme activity were 7 40 °C respectively. stable against values range 6-8. retained 79% its total after incubation one hour at 50 7% (lost 93%) 70 °C. entire completely...

l-Phenylalanine ammonia-lyase (PAL) activity is low in the external layers (flavedo) of intact mature grapefruit peel. Flavedo discs evince upon incubation increasing PAL activity and ethylene production. Light has no effect in enhancing PAL activity in discs. Exogenous ethylene stimulates PAL activity in the flavedo of intact mature grapefruits (half maximum stimulation at 15 ppm); such activi...

Phenylalanine ammonia-lyase (PAL) links the plant primary and secondary metabolisms, its product, trans-cinnamic acid, is derived into thousands of diverse phenylpropanoids. Bambusa oldhamii BoPAL4 has broad substrate specificity using L-phenylalanine, L-tyrosine, L-3,4-dihydroxy phenylalanine (L-DOPA) as substrates to yield p-coumaric caffeic respectively. The optimum reaction pH for three was...

An experiment was conducted to identify the biochemical defense enzymes, viz. catalase (CAT), peroxidase (POX), phenylalanine ammonia lyase (PAL) and total phenol responsible for resistance against A. solani in resistant (cv. H-88-78-1) susceptible (Punjab Chhuhara) cultivars of tomato after application Amistar 25 SC (azoxystrobin 0.1%) during 2015 crop season. Azoxystrobin applied their effica...

Phenylalanine Ammonia-Lyase, Protein Metabolism, Regulation, /ra/js-Cinnamic Acid, Daucus carota, Suspension Culture In vivo and in vitro experiments were performed in order to study the regulatory role of transcinnamic acid and its hydroxylated derivatives (p-coumaric acid, caffeic acid) on the deamina­ tion of phenylalanine catalyzed by PAL (EC 4.3.1.5). 7>arts-cinnamic acid inhibits growth a...