Colmer, Arthur R. (Louisiana State University, Baton Rouge). Relation of the iron oxidizer, Thiobacillus ferrooxidans, to thiosulfate. J. Bacteriol. 83:761-765. 1962.-A restudy has been made of Thiobacillus ferrooxidans secured from the acid drainage of some northern West Virginia coal mines. Cultures isolated from ferrous iron agar and then subcultured for 85 consecutive transfers in thiosulfa...

In the title binuclear complex, [Fe(2)(C(8)H(7)O(2))(4)(C(12)H(8)N(2))(2)(H(2)O)], the Fe(II) ion is six-coordinated by three carboxylate O atoms from three 4-methyl-benzoate ligands, two N atoms from two 1,10-phenanthroline ligands and one bridging aqua ligand in a distorted octa-hedral geometry. The coordinated water mol-ecule acting as the bridging ligand is located on a twofold axes and the...

Introduction: Hydrogen peroxide (H2O2) is a biologically important, non-radical reactive oxygen species (ROS) that can influence several cellular processes. Ability of anti-oxidants to scavenge H2O2 can be measured by several methods but all the methods are suffering from several lacunae due to poor reproducibility, lack of specificity, inaccuracy, high cost etc. Objective: The present study ai...

With the rapid development of information concerning the isolation, identification, and physiological levels of corticosteroids, a relatively simple microchemical met,hod for their quantitative estimation appears ta be desirable. Since the known biologically active adrenal cortical hormones contain a primary cu-ketol function, an cr,@msaturated %-ketone group, or both, several methods based upo...

In a recent publication (1) we reported results obtained by the use of a new analytical procedure for iron in blood plasma. This method, which we find to be more sensitive than those which we have described previously (2-4), depends upon the photoelectric measurement of the red color of the complex formed by ferrous iron with o-phenanthroline. This reaction was first applied, as far as we know,...

We describe an automated colorimetric method for determination of uric acid in serum or urine by use of an AutoAnalyzer II or SMA 12/60 (Technicon Corp.). The method depends on reduction of a ferric-phenanthrolirle complex by uric acid under acidic conditions to a ferrous-phenanthroline complex, which absorbs at 505 nm. Advantages of this method over other methods now in use are that color and ...

1,2-Dihydroxynaphthalene oxygenase was purified from Pseudomonas putida NCIB 9816 grown on naphthalene as the sole source of carbon and energy. The enzyme had a subunit molecular weight of 19,000 and in a medium containing phosphate buffer, 1 mM mercaptoethanol, and 10% (vol/vol) ethanol had a native molecular weight greater than 275,000. The enzyme required Fe2+ for activity. It was inactivate...

Three simple, accurate and economical spectrophotometric methods in ultraviolet and visible region were developed for the determination of cefprozil in bulk and dosage forms have been described. The method A is based on the reduction of ferric ion into ferrous ion by the mentioned drug in the presence of 1,10-phenanthroline to form a highly stable orange red colored ferrion complex measured at ...

With the rapid development of information concerning the isolation, identification, and physiological levels of corticosteroids, a relatively simple microchemical met,hod for their quantitative estimation appears ta be desirable. Since the known biologically active adrenal cortical hormones contain a primary cu-ketol function, an cr,@msaturated %-ketone group, or both, several methods based upo...

In the study of many types of biological material it has become of increasing importance to determine accurately extremely small amounts of iron. Since color reactions are, as a rule, very sensitive, calorimetric procedures are particularly useful in the microanalytical field, and several calorimetric methods have appeared in the literature on iron determination. Although the extremely sensitiv...