Petroleum-hydrocarbon-degrading bacteria were obtained after enrichment on crude oil (as a 'chocolate mousse') in a continuous supply of Indonesian seawater amended with nitrogen, phosphorus and iron nutrients. They were related to Alcanivorax and Marinobacter strains, which are ubiquitous petroleum-hydrocarbon-degrading bacteria in marine environments, and to Oceanobacter kriegii (96.4-96.5 % ...

Natural remediation of oil spills is catalyzed by complex microbial consortia. Here we took a whole-community approach to investigate bacterial incorporation of petroleum hydrocarbons from a simulated oil spill. We utilized the natural difference in carbon isotopic abundance between a salt marsh ecosystem supported by the 13C-enriched C4 grass Spartina alterniflora and 13C-depleted petroleum to...

The diffusive transport of volatile organic compounds (VOCs) in the subsurface at petroleum spill sites can significantly affect vapor migration from sources to buildings and ground surface. Thus, knowledge of compound-specific vapor transport and bio-attenuation is of great interests to those who must identify risks and make corrective action decisions for petroleum spill sites. In this work, ...

The Deepwater Horizon oil spill produced large subsurface plumes of dispersed oil and gas in the Gulf of Mexico that stimulated growth of psychrophilic, hydrocarbon degrading bacteria. We tracked succession of plume bacteria before, during and after the 83-day spill to determine the microbial response and biodegradation potential throughout the incident. Dominant bacteria shifted substantially ...

background heat, chemical and organic pollution are three types of environmental pollution, caused by refinery and petrochemical industries. problems caused by hear and chemical pollutants are currently resolved to some extent but organic pollution such as poly aromatic hydrocarbons (pahs) are still considered as important problems of industry and environment. objectives a laboratory study was ...

The Deepwater Horizon semi-submersible Mobile Offshore Drilling Unit (MODU), leased to BP (previously British Petroleum, now simply “BP”, with the previously-used promotional tagline of “beyond petroleum”) suffered an explosive “blowout” on April 20, 2010 which resulted in what is now the largest offshore oil spill in United States (U.S.) history, and currently estimated to be at least the seco...

Microorganisms that colonize the fuel-in-water emulsion from the Prestige spill have been compared with those from Exxon-Valdez. Both emulsions contained non-fermentative gram-negative rods but unlike Exxon-Valdez’s, the Prestige’s spill contained anaerobic bacteria and no fungi. Our main finding has been the identification of Shewanella putrefaciens, a bacterium promising for bioremediation. T...

Time based observations are the linchpin of improving predictions in any dynamic data driven application systems. Our predictions are based on solutions to differential equation models with unknown initial conditions and source terms. In this paper we want to simulate a waste spill by a water body, such as near an aquifer or in a river or bay. We employ sensors that can determine the contaminan...

In the presented research we have tested the application of photo ionization detector of ECOPROBE 5 analyzer to monitoring of low concentrations of petroleum products in water. Petroleum products are organic pollutants that are present in wastewater most frequently, which is caused by their broad applications and frequent incidents. Emergency leakage of petroleum and its products is predominant...