HTLV-1 causes adult T-cell leukemia (ATL) and HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP). Recombinant envelope glycoprotein is used in production of diagnostic enzyme-linked immunosorbent assay (ELISA) kit. There are some reports that a significant percentage of Iranian HTLV-1 infected patients showed no seroreactivity with MTA-1 peptide, while HTLV-1 had been confirmed...

Endoglucanase is an important enzyme participating in the hydrolysis of cellulose into oligosaccharides or glucoses can be used a variety industrial fields. A gene (code GL0183420 designated as eg9) 1398 bp encoding for endoglucanase family GH8 was identified from metagenomic DNA data bacteria humus surrounding wood hydrolyzed by white rot fungi. The 126 terminal nucleotides at 5' encode signal...

Glycolate oxidase is a peroxisomal flavoprotein catalyzing the oxidation of glycolate to glyoxylate and plays crucial metabolic roles in green algae, plants, animals. It could serve as biocatalyst for enzymatic production glyoxylate, fine chemical with wide variety applications perfumery, flavor, pharmaceutical agrochemical industries. However, low catalytic activity native levels active enzyme...

Các hạt silica (SiO2) được cố định protein là vật liệu đầy triển vọng trong các lĩnh vực y sinh hay cảm biến học. Những nghiên cứu trước cho thấy ribosome L2 của vi khuẩn Escherichia coli liên kết mạnh với silica. Ngoài ra, A thụ thể bề mặt có tính ổn cao, nguồn gốc từ Staphylococcus aureus, sự giữa và kháng xem một những tương tác protein-protein kinh điển nhiều nhất hiện nay. Nghiên này tiến ...

The Cry (crystal) proteins from Bacillus thuringiensis are known to have toxicity against a variety of insects and have been exploited to control insect pests through transgenic plants and biopesticides. B. thuringiensis SBS BT-1 carrying the cry2 genes was isolated from soil samples in Pakistan. The 2-kb full length cry2Ac gene was cloned, sequenced, and submitted to the EMBL DNA database (Acc...

Clostridium perfringens beta toxin is only produced by types B and C and plays an important role in many human and animal diseases, causing fatal conditions that originate in the intestines. We compared the expression of C. perfringens type B vaccine strain recombinant beta toxin gene in the Escherichia coli strains RosettaTM(DE3) and BL21(DE3). The beta toxin gene was extracted from pJETβ and ...

Protease 2A (2Apro) of coxsackievirus B3 (CVB3) plays a major role in viral replication. In case of infection, viral proteins are being synthesized from viral mRNA using host biosynthesis machinery. 2Apro of virus, after being synthesized, exhibits two critical functions, cleavage of viral proteins and breaking eukaryotic initiation factor 4G. The enzyme plays an essential role in viral replica...

OBJECTIVES Tuberculosis (TB) is still one of the problematic infectious diseases in developing countries, especially in Iran. In the present study, we applied ribosome display technique to select single chain variable fragments (scFvs) specific for the 6-kDa early secretory antigenic target (ESAT-6) antigen of Mycobacterium tuberculosis from a mouse scFv library. MATERIALS AND METHODS The gen...

Hypothetical proteins are functionally uncharacterized proteins with assigned function using sequence annotation tools. Almost half of the coding regions of several genomes are hypothetical proteins. Therefore, it is of our interest to characterize a hypothetical protein YVRE from the model system Bacillus subtilis using known data. YVRE is assigned the function as a glucono-lactonase using pre...

Hypericum perforatum is well known for its antidepressant and anti-inflammatory activities, for which hypericin and its derivatives are indicated to be the most active compounds. Hypericin synthase (Hyp-1) is the only protein proven to catalyze the synthesis of hypericin. In this study, the full-length cDNA of Hyp-1 was chemically synthesized according to the Hyp-1 sequence in GenBank (accessio...