Chemiluminescence (CL) is a very promising method for applications in analytic chemistry and biological systems due to the elimination of external light source, which could reduce autofluorescence interference improve signal-to-noise ratio (SNR) tissue penetration depth. The fluorescent dyes, chemiluminescent initiator, reaction temperature are three crucial factors that determine chemiluminesc...

Catecholamines, namely, dopamine, norepinephrine and epinephrine, play important roles in higher animals as neurotransmitters or hormones, and are metabolized by catechol-O-methyltransferase (COMT). To elucidate the role of COMT in blood pressure regulation, we have developed simultaneous determination methods for catecholamines and their 3-O-methyl metabolites using high-performance liquid chr...

A new formal strategy in the multidimensional optimisation of the experimental variables affecting the chemiluminescence (CL) detection in flow injection analysis (FIA) is proposed here. The strategy implies several steps, being the most significant: selection of the variables to be studied and their experimental domain; use of a screening design to detect significant variables and interactions...

A fully automated and highly sensitive method with a semi-microcolumn liquid chromatography system for the determination of rat plasma catecholamines (CAs) was developed. Automated on-line extraction of CAs in diluted plasma using a precolumn packed with strong acidic cation exchange resin was coupled with separation of CAs on a semi-microcolumn (250 x 1.5 mm id). fluorogenic derivatization wit...