conclusions in conclusion, occult hbv was found in 36% of patients with negative results for hbsag, but positive results for hcv. detection of hbv-dna in both pbmcs and plasma together in comparison with plasma alone provided more true identification of obi.the svr rate was significantly higher in coinfected patients than mono-infected ones. background occult hepatitis b virus (hbv) infection (...

Impact of replacing dietary saturated with unsaturated fats on the expression genes related to cholesterol metabolism in peripheral blood mononuclear cells: Findings from RISSCI-1 study - Volume 80 Issue OCE5

cryopreservation is the method of choice for long term storage of human pbmcs. this study was designed to compare the different combinations of variables affecting the cryopreservation of pbmcs samples. the viability of pbmcs separated from 2×5 ml peripheral blood samples obtained from 16 healthy adult volunteers, were measured using trypan blue dye exclusion method just before freezing with di...

mitochondria play key roles in many cell functions including energy production, fatty acid metabolism, pyrimidine biosynthesis, calcium homeostasis, and aging. they also regulate crucial signaling cascades such as apoptosis and oxidative stress. the proteome is often used to investigate the functional correlations on protein levels. based upon the human, genome there is estimated 2000 to 2500 a...

Th17 response following in vitro exposure to mercury with and without n-3 long chain polyunsaturated fatty acids peripheral blood mononuclear cells from systemic lupus erythematosus patients compared healthy controls - Volume 82 Issue OCE4

Background and Objectives: The mesenchymal stem cells derived from peripheral blood (PB) have been recognized as a promising source for allogeneic cell therapy. The aim of this study was to investigate the isolation, growth and differentiation ability of peripheral blood-isolated mesenchymal stem cells. Methods: The mononuclear cells were purified from fr...

Background: Colorectal cancer (CRC) is the third most common cancer worldwide. Studies have indicated that immune cells and soluble factors play a key role in maintaining the balance between tumor-promoting inflammation and anti-tumor immunity. It has been shown that secreted cytokines from CRC cell lines could affect peripheral blood mononuclear cells (PBMCs), monocytes, and macrophages phenot...

angiocardiography is an x-ray examination of the blood vessels or chambers of the heart. cardiologists and staff members applying this procedure are exposed to high levels of scattered radiation. in our previous study the incidence of unstable chromosomal aberrations and cytokinesis-blocked micronuclei were found to be significantly higher in exposed individuals than the age and sex matched con...

background: dendritic cells (dcs) are the most potent stimulators of primary t cell responses and play a key role in immune reactions after stem cell transplantation. very little is known about the cord blood (cb) dendritic cells and their potential involvement in the low incidence and lower severity of acute graft-versus-host disease after cb transplantation.   objectives: the aim of this stud...