The 5 S fragments of rabbit immunoglobulin G were prepared by digestion with pepsin or with CNBr. Cleavage of these fragments with trypsin led to the localization of the positions of these splits on the heavy chain. The COOHterminal residue released by carboxypeptidase A from the papsin-produced 5 S fragment was leucine. The pepsin-5 S fragment was reduced and aminoethylated to yield the 3.5 S ...

1. Collagen was extracted from chick skin with dilute acetic acid followed by dilute acetic acid containing pepsin. 2. The solubilized collagens were purified and portions subjected to further digestion by pepsin. 3. This treatment decreased the aldehyde content but contamination by hexosamine was not diminished. 4. Pepsin treatment converted practically all the acid-soluble collagen into monom...

1. A quantitative method for the determination of pepsin is described depending on the change in conductivity of a digesting egg albumin solution. 2. The combination of pepsin with an insoluble substrate has been followed by this method. 3. The amount of pepsin removed from solution by a given weight of substrate is independent of the size of the particles of the substrate. 4. There is an optim...

Gnathostoma spinigerum third stage larvae antigen is necessary for Western blot analysis in the diagnosis of Gnathostomiasis. Acid pepsin solution is required for digestion of eel's liver( Fluta alba ) to yield the larvae for antigen preparation. Here, he reported the feasibility of using orange juice as an alternative to enzyme pepsin in harvesting Gnathostoma spinigerum third stage larvae. Fr...

The wide-spread distribution of trypsin inhibitors throughout the vegetable and animal kingdoms (1) makes elucidation of their physiological function of great interest. From this point of view, resistance to peptic digestion becomes an important property, since only an inhibitor resistant to pepsin is likely to produce any physiological effect when ingested by a normal animal. The soy bean inhi...

The results of immunofluorescence studies in nine cases of rheumatoid arthritis and nine cases of ankylosing spondylitis are presented. Antiglobulin activity was sought with fluorescein labelled, heat aggregated human IgG and rabbit immunoglobulin (indirect immunofluorescence). A similar degree of increased staining for rheumatoid factor activity was seen after pepsin digestion in all cases of ...

Collagen from human skin was fractionated into neutral salt-soluble, acid-soluble, pepsin-released, and insoluble fractions. No age-related changes were observed in the proportion of collagen extracted by neutral salt. A significant age-related decrease in the proportion of acid-soluble collagen was found. A highly significant (P less than 0.001) age-related decrease in the amount of collagen r...