Among 117 of the albino mice used in this study, 108 were intraperitionally infected with 100 X 106 promastigotes of Leishmania donovani (MHOM\ IQ\ 982\ BRCI) strain, while the rest groups (9) were left without infection. The inoculation of albino mice caused the elevation of liver and spleen weight after 7-15 days and the treated mice with 20 mg/kg by Pentostam and Allupurinol for 10 and 15 da...

The standard treatment of human visceral leishmaniasis involves the use of pentavalent antimony (Sb(V)). Its mechanism of action is unknown because of the limited information available about intracellular antimony metabolism and about the genes that regulate these processes. Herein, flow injection-inductively coupled plasma mass spectrometry (ICP-MS), flow injection hydride generation ICP-MS, a...

BACKGROUND & OBJECTIVES Several plant products have been tested and found to possess antileishmanial activity. The present study was undertaken to establish whether methanolic extract of Allium sativum Linn has antileishmanial activity in comparison to standard drugs. METHODS Methanolic extract of A. sativum bulbs was screened for in vitro and in vivo antileishmanial activity against Leishman...

Leishmania tarentolae promastigotes were selected step by step for resistance to sodium stibogluconate (Pentostam). Mutants resistant to antimony-containing drugs and cross-resistant to arsenite were therefore obtained. Amplification of one common locus was observed in several independent sodium stibogluconate-resistant mutants, and the locus amplified was novel. The copy number of the amplifie...

Pentavalent antimony, as meglumine antimoniate (Glucantime) or sodium stibogluconate (Pentostam), is the main treatment for leishmaniasis, a complex of diseases caused by the protozoan Leishmania, and an endemic and neglected threat in Brazil. Despite over half a century of clinical use, their mechanism of action, toxicity and pharmacokinetic data remain unknown. The analytical methods for de...