The Pastorex Aspergillus antigen test for detection of Aspergillus galactomannan antigen in the sera of patients with invasive aspergillosis is used in many clinical laboratories. A serum sample contaminated with Penicillium chrysogenum gave a strongly positive reaction (1:128) which was heat stable, was not eliminated by pronase treatment, and was not detected by a normal rabbit globulin contr...

The soil samples were collected and were primarily screened for isolation of amylase producing fungi. Among the isolated fungi, amylase producing isolates were identified by growing on starch agar media. The isolates exhibiting the maximum zone of clearance on starch agar media by iodine were identified and were subcultured on potato dextrose agar (PDA). The isolates were morphologically charac...

Penicillium chrysogenum secretes a low molecular weight, cationic and cysteine-rich protein (PAF). It has growth inhibitory activity against the model organism Aspergillus nidulans and numerous zoo- and phytopathogenic fungi but shows only minimal conditional antifungal activity against the producing organism itself. In this study we provide evidence for an additional function of PAF which is d...

The development and study of strains of Penicillium chrysogenum that give high yields of penicillin has been a major project at the University of Wisconsin during the past 12 years. Many thousands of cultures have been screened for their penicillin-producing ability in the Botany Department of the University. The methods used for the production and screening of mutants, the genealogy of the out...

Studies on the mechanism of glucose metabolism by penicillia are few. The fact that Snell (1949) and Kita (1951) found none of the phosphorylated esters of the Embden-Meyerhof scheme in PeniciUium chrysogenum, strain Q176, and that penicillia contain glucose oxidase (Birkinshaw and Raistrick, 1943) would indicate a priori that glucose is metabolized oxidatively by the mold. The following data i...

The Penicillium chrysogenum antifungal protein PAF inhibits the growth of various filamentous fungi. In this study, PAF was found to localize to the cytoplasm of sensitive aspergilli by indirect immunofluorescence staining. The internalization process required active metabolism and ATP and was prevented by latrunculin B, suggesting an endocytotic mechanism.

BACKGROUND The genus Penicillium contains a large number of ubiquitous environmental taxa, of which some species are clinically important. Identification of Penicillium down to the species level is currently based on polyphasic criteria, including phenotypic features and genetic markers. Biodiversity of the genus Penicillium from Mazandaran and Tehran provinces has not been described. OBJECTI...

A highly inducible fungal promoter derived from the Penicillium chrysogenum endoxylanase (xylP) gene is described. Northern analysis and the use of a beta-glucuronidase (uidA) reporter gene strategy showed that xylP expression is transcriptionally regulated. Xylan and xylose are efficient inducers, whereas glucose strongly represses the promoter activity. Comparison of the same expression const...

Since the aflatoxins were first reported in 1961 from Aspergillus flavus, mycotoxins have often been named after the fungus which was first found to produce them. A long list of connections between fungal species and mycotoxins and antibiotics has been reported, but unfortunately many of the identifications, and hence the connection between mycotoxin name and the source of the toxin, are incorr...

Indole-3-acetonitrile obtained from cabbage has been found to inhibit growth of Pyrausta nubilalis (Hbn.), Galleria mellonella (L.), and Penicillium chrysogenum.