Extracellular penicilhnase from Bacillus cereus 569 was found to exist in two states, an iodine-sensitive state and an iodine-insensitive state. Iodine-sensitive penicillinase is inactivated by 2.5 X 10-a M iodine within 1 min, while the iodine-insensitive enzyme is inactivated at a much slower rate. Approximately 20% of the extracellular penicillinase activity found in crude or partially ptied...

Penicillinase from Bacillus cereu.s 569 was purified and examined for homogeneity by sedimentation analysis, aminoterminal analysis, and vertical acrylamide gel electrophoresis. The enzyme preparation was shown to contain three distinct species of extracellular penicillinase which could be separated easily by gel electrophoresis. Kinetic studies failed to reveal any significant differences in t...

The penicillinase of Bacillus cereus exists as an exoenzyme, called cw-penicillinase, and as an immunologically distinct cellbound enzyme, designated as y-penicillinase (1). Properties such as substrate specificity, Michaelis constant, and sedimentation rate were found to be practically identical, and the only other ways in which the cell-bound y-penicillinase differed from LYpenicillinase were...

It is known that penicillin resistance of bacteria is mainly caused by the inactivation of penicillin by penicillinase derived from such strains. We have developed a screening procedure for penicillinase inhibitors. Several microorganisms were found to produce such inhibitors, and from the culture filtrate of Streptomyces gedanensis ATCC 4880 a penicillinase inhibitor, named KA-107, was isolate...

Saz, Arthur K. (National Institute of Allergy and Infectious Diseases, Bethesda, Md.), Dolores L. Lowery, and Leah J. Jackson. Staphylococcal penicillinase. I. Inhibition and stimulation of activity. J. Bacteriol. 82:298-304. 1961.-The penicillinase extracted from a penicillin-resistant strain of Staphylococcus aureus was shown to be inhibited up to 70% by various dipeptides and particularly by...

an enzyme-linked immunosorbent assay (elisa) for progesterone measurement in serum or plasma samples using penicillinase as label enzyme is reported. a c3 and c11 derivatives of progesterone were prepared and conjugated to bovin serum albumin (bsa). polyclonal antibody against these two immunogens were prepared in new zealand white rabbits. purified ig fractions of antibodies were immobilized o...

Delay in the appearance of Neisseria gonorrhoeae on Thayer-Martin medium was associated with delay in incubation and with the use of urine samples from infected men for cultural diagnosis. Self-treatment among women was related to inhibition of growth of N gonorrhoeae, particularly of non-penicillinase-producing gonococci (beta-lactamase). The difference in the time of appearance of penicillina...

Harmon, Shirley A. (Ohio State University, Columbus), and Jack N. Baldwin. Nature of the determinant controlling penicillinase production in Staphylococcus aureus. J. Bacteriol. 87:593-597. 1964.-From three penicillinase-producing strains of Staphylococcus aureus 38 penicillin-sensitive mutants were isolated. With phages 80 and 53 of the International Typing Series, each of these mutants was a ...

A screening procedure was used to isolate a number of mutants of Escherichia coli K-12 with low penicillinase activity. By co-transduction with purA, three of the mutants were found to map near 82 min. Penicillinase was purified from one mutant and from a transductant with a temperature-sensitive enzyme. Comparison with wild-type penicillinase revealed similarities in the Ouchterlony immunodiff...