BACKGROUND Mast cell and basophiles are thought to be central to inflammation that has an allergic basis as allergens activate these cells in an IgE-dependent manner to generate mediators such as histamine, eicosanoids and cytokines. Phosphodiesterase (PDE) is known to exist as multiple molecular forms of enzyme that metabolise the second messengers. Studies of our own have shown that, of a var...

Cyclic nucleotide phosphodiesterase (PDE) isoforms can influence disease pathogenesis and be novel therapeutic targets. Because lower cAMP levels may contribute to the decreased apoptosis that occurs in chronic lymphocytic leukemia (CLL), we assessed the expression levels of PDE isoforms in peripheral blood mononuclear cells (PBMC) of healthy adults and patients with CLL. We found a unique PDE ...

A cDNA clone corresponding to a novel cGMP phosphodiesterase inhibitory subunit (PDE gamma) was isolated from a bovine retinal cDNA library using a rod PDE gamma cDNA as a probe under conditions of reduced stringency. This new clone encodes an 83-amino acid protein approximately 80% identical to rod PDE gamma. The transcript for this protein is retina specific and 940 base pairs in length. Sout...

Type V phosphodiesterase (PDE V) metabolizes cyclic guanosine monophosphate (cGMP) and is abundant in the kidney and vasculature and was found recently in the heart. Sildenafil is a PDE V inhibitor that is used clinically for erectile dysfunction. Brain natriuretic peptide (BNP) is a cardiac peptide with vasodilating, lusitropic, and natriuretic properties that are mediated via cGMP. It was hyp...

On the basis of our study on the structure-activity relationships of 1,3,7-alkylxanthines and condensed-purines on cAMP-phosphodiesterase 4 (PDE 4) isoenzyme inhibitor, we investigated the synthesis and the inhibitory activity of 3-phenylxanthine and 4-phenyl[i]condensed-purine derivatives. Xanthines and condensed-purines with the phenyl group exhibited potent and selective PDE 4 inhibitory act...

background: pregnancies with early onset fetal growth restriction have poor perinatal outcome. sildenafil citrate (pde -5 inhibitor) as a vasodilator increases uteroplacental blood flow and potentiates fetal growth. case presentation: in this study, a case was examined and sildenafil was administered for her. it was found that sildenafil improved the uterine blood flow with a favorable fetal ou...

in this study, we aimed to investigate the peripheral antinociception effects of specificphosphodiesterase 5 (pde-5) inhibitor vardenafil on carrageenan-induced nociception in rats,and the role of calcium besides the l-arginine- nitric oxide (no)- cyclic guanosine monophophate(cgmp) pathway in these effects. hyperalgesia was induced by the intraplantar injection of 0.1ml fresh carrageenan solut...

AIM To investigate which phosphodiesterase (PDE) is involved in regulating cyclic 3'5' guanosine monophosphate breakdown in retinal pigment epithelium (RPE) cells. METHODS cGMP content in the cultured RPE cells (D407 cell line) was evaluated by immunocytochemistry in the presence of non-selective or isoform-selective PDE inhibitors in combination with the particulate guanylyl cyclase stimulat...

We report our initial experience with salvage therapy for low responders to PDE-5 inhibitors by adding vitamin E. Of 89 patients with ED who visited our clinic between January 2004 to August 2006, 9 were unable to obtain a full response to a PDE-5 inhibitor and included in the present study. After providing informed consent, each was given 300 mg per day of alpha-tocophenol at least 1 month and...