نتایج جستجو برای: pcrrflp

تعداد نتایج: 65  

Journal: :The Indian journal of medical research 2008
D Bhattacharya A K Bera B C Bera D Pan S K Das

BACKGROUND & OBJECTIVE Cystic echinococcosis (CE) has a wide host range and distinct entities, not only reflected phenotypically but also by genotypic variation. Considering this fact, this study was undertaken to characterize the Indian isolates of Echinococcus granulosus to find out difference between Indian cattle, buffalo and sheep isolates on the basis of random amplification of polymorphi...

2002
DAVID B. TAYLOR RICHARD D. PETERSON JAMES J. PETERSEN

Ann. EntomoI. Soc. Am. 90(6): 814-824 (1997) ABSTRACT Polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) and sequencing analyses were used to characterize an amplicon of -625 bp in 4 of the 5 nominate species of Muscidijilrax Girault & Sanders, pupal parasitoids of muscoid Hies. A single polymorphic nucleotide site was observed among 2 samples of M. raptOr Girault & Sa...

Journal: :Asian Pacific journal of cancer prevention : APJCP 2011
Junxiang Chen Meichu Cheng Li Yi Chang-bin Jiang

AIM To study the potential role of cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1) polymorphisms in the risk of renal cell cancer in Chinese. METHODS A total of 181 pathologically-proven renal cancers and 350 controls from the second Xiangya Hospital in Changsha were collected during the period from May 2007 to December 2010. CYP1A1 genetic polymorphisms were genotyped using PC...

2013
Srinivasan Muthuswamy Shally Awasthi Pratibha Dixit Sarita Agarwal Sanjay Gandhi

Background: Involvement of impaired folate metabolism has been hypothesized to be a potential risk factor for the predisposition of asthma. The reports are conflicting on the potential association between atopic disease and polymorphism of the methylene-tetrahydrofolate reductase (MTHFR)-gene. Objective: We designed the study to investigate the association of C677T polymorphism of the MTHFR gen...

Journal: :iranian journal of animal biosystematics 0
b. sahebjam j. darvish e. rastegar-pouyani r. siahsarvie s. akbarirad

the calomyscidae is a small family of rodentia that has recently been classified into 8 species in a single genus, calomyscus. currently 5 species are reported in iran: c. elburzensis,، c. bailwardi، c. grandis ، c. urartensis and c. hotsoni. species discrimination based on morphological characteristics is difficult, so this study re-evaluated the phylogenetic relationships and taxonomic status...

2017
Weidong Hu Yun Chen Heqing Zhao

The progressive neurodegenerative process in Parkinson’s disease (PD) is accompanied by chronic inflammation, including activation of microglia and astrocytes that express pro-inflammatory cytokines. Up to now, abundant evidence from genetic epidemiology have been assessed the association of pro-inflammation cytokines gene polymorphisms and risk of PD in different ethnicity, but conflicting res...

2012
Roger Wumba Menotti Jean Longo-Mbenza Benjamin Mandina Madone Kintoki Fabien Zanga Josué Sala Jean Kendjo Eric Guillo-Olczyk AC Thellier Marc

Objective. To determine the prevalence and the genotypes of Enterocytozoon bieneusi in stool specimens from HIV patients. Methods. This cross-sectional study was carried out in Kinshasa hospitals between 2009 and 2012. Detection of microsporidia including E. bieneusi and E. intestinalis was performed in 242 HIV-infected patients. Typing was based on DNA polymorphism of the ribosomal DNA ITS reg...

2017
Liping Jiang Jingming Wang Xiang Gao Shuzhen Liu

Background: Gastric cancer (GC) is one of the most common malignancies worldwide. To date, the polymorphisms in the promoter region of tumour-necrosis factor-α (TNF-α) gene have been extensively studied in relation to different kinds of cancer, including GC. Methods and materials: In this case-control study, the case population consisted of 240 GC cases and 510 healthy controls. Genotyping of T...

Journal: :Clinical chemistry 2004
John Shepherd Brian Baldwin

with the replacement of A1298C wild-type reverse primer with A1298C mutant reverse primer, which had the following sequence: 5 -GGTAAAGAACAAAGACTTCAAAGACACTGTG-3 (Sigma-Aldrich). ARMS PCR conditions were 95 °C for 5 min; 40 cycles of 95 °C for 30 s, 57 °C for 30 s, and 72 °C for 30 s; and 72 °C for 5 min. The wild-type reaction produces a 120-bp product, whereas the mutant reaction produces a 1...

2013
Lucia Procopciuc Gabriela Caracostea Georgiana Iordache Ileana Olteanu F. Stamatian

Objectives. 1. to determine the genotype distribution of Met235Thr, Thr174Met, insertion (I)/deletion (D) in the ACE gene, 18-83G/A in the REN gene, 1166A/ C in the AGTR1 gene and 3123A/G in the AGTR2 gene in women with and without PE (preeclampsia); 2. to examine the possible influence of RAS gene polymorphisms in the development and perinatal outcome of PE. Methods. Forty-five women with PE w...

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