The emergence of self-replication and information transmission in life’s origin remains unexplained despite extensive research on the topic. A hypothesis explaining the transition from a simple organic world to a complex RNA world is offered here based on physical factors in hydrothermal vent systems. An interdisciplinary approach is taken using techniques from thermodynamics, fluid dynamics, o...

Valasek, Mark A., and Joyce J. Repa. The power of real-time PCR. Adv Physiol Educ 29: 151–159, 2005; doi:10.1152/advan. 00019.2005.—In recent years, real-time polymerase chain reaction (PCR) has emerged as a robust and widely used methodology for biological investigation because it can detect and quantify very small amounts of specific nucleic acid sequences. As a research tool, a major applica...

� Ensure reproducibility of the clean-up procedure Properly purified PCR products are crucial for many molecular biological applications. When a large number of different products are required the purification procedure has to deal with many samples in parallel. Moreover, it needs to be fast and reliable. Authors: Dr. Thomas Zinn 1and Dr. Martin Gajewski 2 1 MACHEREY-NAGEL GmbH & Co. KG, Neuman...

Real-time polymerase chain reaction (PCR) is being used worldwide for various research purposes. In this review we discuss the principles of real-time PCR, different methodologies and chemistries available and their applications in mycobacterial research. This technology allows for the direct detection of PCR product during the exponential phase of the reaction. Being a very powerful, accurate,...

This book covers general challenges of introducing primarily noncommercial PCRs and specific procedures into the laboratory, including sample treatment, extraction protocols, quality and quality assurance, and internal and external laboratory processes. The chapters on specific pathogens illustrate principles that could be applied in many diagnostic laboratories. The editor’s preface to this bo...

The polymerase chain reaction (PCR) is a rapid scientific method for generating a 106-107fold increase in the number of copies of discrete DNA or RNA sequences (Boehm,1989; Imboden et al,1993). The use of PCR technology has greatly increased the ability to study on genetic material. PCR is a rapid and reliable molecular biology technique that allows quick replication of mainly DNA, the starting...