نتایج جستجو برای: pcr multiplex

تعداد نتایج: 186945  

2007
Mary C. Edwards Richard A. Gibbs

Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas 77030 Ever since it was shown that PCR could simultaneously amplify multiple loci in the human dystrophin gene, (1) multiplex PCR has been firmly established as a general technique. A short list of multiplex PCR applications now includes pathogen identification, gender screening, linkage analysis, forensic studies, tem...

D. Brahma D. Narang, M. Chandra S. T. Singh

Background: Paratuberculosis and tuberculosis (TB) caused by Mycobacterium avium paratuberculosis (MAP) and Mycobacterium tuberculosis complex (MTC), respectively are economically important, chronic debilitating diseases affecting the dairy herds and are also potential zoonotic threats. Aims: Differential diagnosis of paratuberculosis and TB i...

Journal: :Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 2003
Donna J Johnson Eileen N Ostlund Beverly J Schmitt

A traditional nested reverse transcription-polymerase chain reaction (RT-PCR) assay specific for eastern equine encephalomyelitis (EEE) virus was designed to multiplex with a previously described West Nile (WN) virus nested RT-PCR assay. Differentiation of EEE and WN was based on base pair size of the amplified product. One hundred fifty-seven mammalian and avian brain tissues were tested by EE...

ژورنال: :پژوهش های آسیب شناسی زیستی 0
mojtaba saadati associated professor, department of biology, imam houssein university, tehran, iran shahram nazarian m.sc., department of biology, imam houssein university, tehran, iran babak barati m.sc., department of biology, imam houssein university, tehran, iran hossein mehdizadeh m.sc., department of biology, imam houssein university, tehran, iran hadi shirzad shirzad ph.d. student, department of biology, imam houssein university, tehran, iran majid sadeghizadeh associated professor, department of genetics, faculty of basic sciences, tarbiat modares university, tehran, iran abbas mosapour

هدف: مننژیت باکتریایی یکی از عفونت های جدی و گاهی کشنده است که روی سیستم اعصاب مرکزی تأثیر می گذارد. دانستن علت مننژیت ایجاد شده توسط عوامل ویروسی یا باکتریایی، به دلیل تفاوت در شدت بیماری و نیز درمان آن از اهمیت خاصی برخوردار است زیرا آنتی بیوتیک ها می توانند مانع از گسترش بعضی از این عوامل در بین مردم شوند. نیسریا مننژیتیدس و هموفیلوس آنفولانزا دو عامل مهم بیماری زا هستند که در ایجاد مننژیت ح...

ژورنال: :مجله دانشگاه علوم پزشکی شهرکرد 0
آرزو کمالی arezo kamali sirjanbranch, islamic azad universityدانشگاه آزاد اسلامی، سیرجان کیومرث امینی kumarss amini saveh branch, islamic azad universityدانشگاه آزاد اسلامی، ساوه

زمینه و هدف: سودوموناس آئروژینوزا یک پاتوژن فرصت طلب و عامل عفونت های بیمارستانی و مرگ و میر به خصوص در بیماران با ضعف سیستم ایمنی می باشد و مقاومت ذاتی به مواد ضد میکروبی منجر به مشکلاتی در درمان عفونت های آن می شود. هدف از این مطالعه بررسی فراوانی ژن های حدت سودوموناس آئروژینوزا به روش multiplex-pcr می باشد. روش بررسی: 60 نمونه سودوموناس آئروژینوزا شامل 40 نمونه از کلکسیون آزمایشگاه میکروبیول...

Journal: :veterinary research forum 2015
asma afshari abdollah jamshidi jamshid razmyar mehrnaz rad

clostridium perfringens is an important cause of bacterial food poisoning worldwide. the disease is caused by c. perfringens enterotoxin (cpe) encoded by cpe gene. the aim of this research was to identify the different types of c. perfringens and the presence of cpe gene in isolated bacteria from broilers’ meat marketed in retail meat shops of mashhad city in northeastern of iran. after isolati...

Journal: :archives of pediatric infectious diseases 0
mohammad fateh amirkhiz department of microbiology, faculty of medicine, tabriz university of medical sciences, tabriz, ir iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (tabriz university of medical sciences) mohammad ahangarzadeh rezaee infectious and tropical diseases research center, tabriz university of medical sciences, tabriz, ir iran; infectious and tropical diseases research center, tabriz university of medical sciences, tabriz, ir iran. tel: +98-41-33364661, fax: +98-41-33364661سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (tabriz university of medical sciences) alka hasani department of microbiology, faculty of medicine, tabriz university of medical sciences, tabriz, ir iran; infectious and tropical diseases research center, tabriz university of medical sciences, tabriz, ir iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (tabriz university of medical sciences) mohammad aghazadeh department of microbiology, faculty of medicine, tabriz university of medical sciences, tabriz, ir iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (tabriz university of medical sciences) behrooz naghili infectious and tropical diseases research center, tabriz university of medical sciences, tabriz, ir iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (tabriz university of medical sciences)

conclusions as about 70% of methicillin-resistant isolates belonged to sccmec iii, the present study can conclude that, over an 8-year period, only one dominant and stable clone of mrsa strain was found in tabriz hospitals. this finding could be a result of incorrect medical orientations, inadequate infection controlling policies, and insufficient preventive approaches. objectives the current s...

Journal: :Turkish journal of medical sciences 2016
Cheronie Shely Stanis Beng Kah Song Tock Hing Chua Yee Ling Lau Jenarun Jelip

BACKGROUND/AIM Malaria is a major public health problem, especially in the Southeast Asia region, caused by 5 species of Plasmodium (P. falciparum, P. vivax, P. malariae, P. ovale, and P. knowlesi). The aim of this study was to compare parasite species identification methods using the new multiplex polymerase chain reaction (PCR) against nested PCR and microscopy. MATERIALS AND METHODS Blood ...

Journal: :iranian journal of public health 0
reza ranjbar molecular biology research center, baqiyatallah university of medical sciences, tehran, iran. davoud afshar dept. of pathobiology, school of public health, tehran university of medical sciences, tehran, iran. ali mehrabi tavana health management research center, baqiyatallah university of medical sciences, tehran, iran. ali najafi molecular biology research center, baqiyatallah university of medical sciences, tehran, iran. fatemeh pourali molecular biology research center, baqiyatallah university of medical sciences, tehran, iran. zahra safiri molecular biology research center, baqiyatallah university of medical sciences, tehran, iran.

shigella species are among the common causes of bacterial diarrhoeal diseases. traditional detection methods are time-consuming resulting in delay in treatment and control of shigella infections thus there is a need to develop molecular methods for rapid and simultaneous detection of shigella spp. in this study a rapid multiplex pcr were developed for simultaneous detection of three pathogenic ...

Journal: :journal of medical microbiology and infectious diseases 0
azam fatahi ibn sina street,isfahan,isfahan health center ali ajami ibn sina street,isfahan,isfahan health center mojgan bozorgzad ibn sina street,isfahan,isfahan health center shahram nekoeian ibn sina street,isfahan,isfahan health center atefeh khazeni ibn sina street,isfahan,isfahan health center

introduction: awareness of the species of shigella has particular importance in the way of dealing with an outbreak, controlling it, and treating patients. the purpose of this study was to use the multiplex pcr method and comparison with the culture method to detect shigella species, as well as analysing the antibiotic resistance pattern in its different species. methods: simultaneously, the fe...

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