Introduction. Bacillus anthracis is the causative agent of anthrax, a pathogen characterized by high genetic monomorphism that complicates differentiation strains. Thus, molecular methods for typing require improvement.
 The aim study. To select marker SNPs new groups B. and to develop method their laboratory identification using HRM PCR.
 Materials methods. core genome 222 strains fr...

Introduction. Polymerase chain reaction (PCR)-based diagnostic tests use purifi ed nucleic acids (NAs) from clinical samples. The NAs cation step adds time, cost, and aff ects the quality of testing. objective this study was to develop a protocol for direct saliva in genetic markers, without acids. Methods. PCR, real-time RT-PCR isothermal amplifi were used detection Results. We report markers ...

The aim of this study was to identify the cell surface cluster of differentiation (CD) markers of the cell lines infected by Theileria annulata schizont. The CD molecules are very useful for the characterization of cells and different subpopulations of leukocytes. They are usually recognized by specific antibodies using flow cytometry and immunohistochemistry. In the current stud...

The most significant pulse crop in India is the mungbean [Vignar adiata (L.) Wilczek], which constitutes almost 16% of national area. For biotechnological interventions, it prerequisite to develop a rapid and cost-effective method for genomic DNA isolation suitable PCR. In present investigation,modified CTAB was used extract from leaf tissues mungbesn. By crushing leaves several genotypes pre-h...

DNA molecular weight standard control, also called DNA marker (ladder), has been widely used in the experiments of molecular biology. In the paper, we report a method by which DNA marker was prepared based on multiple PCR technique. 100-1000 bp DNA fragments were amplified using the primers designed according to the 6631 ~ 7630 position of lambda DNA. Target DNA fragments were amplified using T...

Background: Downy mildew caused by Plasmopara halstedii is one of the most devastating diseases of sunflower worldwide. So far several dominant resistance genes designated as Pl have been identified and their molecular markers linked to these genes are available. However, no information on the resistance genes is available in Iranian lines. Objective: In this study, the presence of three map-b...