the protective effect of a l-type calcium channel blocker, nimodipine, on cell injury induced by oxygen-glucose deprivation (ogd) in pc12 cells was investigated. pc12 cells were exposed to in-vitro oxygen-glucose deprivation (30 minutes and 60 minutes respectively) in the presence or absence of nimodipine (10mm/l) in three different time schedules (pre-24h, pre-3h and concurrently). cellular vi...

background and objective: in neurodegenerative disorders, oxidative stress mediated by reactive oxygen species is strongly associated with increased neuronal damages that lead to apoptosis. pro-apoptotic and anti-apoptotic gene expressions were changed during cell differentiation that affect cell viability and differentiation. this study was conducted to determine the effects of hydrogen peroxi...

micrornas (mirnas) are a class of small non coding regulatory rnas that have key functions in multiple cell processes. deregulation of these tiny mirnas are involved in various human diseases. mir-155 is one of the multifunctional mirna that its over-expression has been found to be associated with different kinds of cancer such as leukemia, breast and colon cancers. it is thought that deregulat...

application of nanotechnology in medicinal and biological fields has attracted a great interest in the recent yeras. in this paper the cell membrane leakage induced by iron nanoparticles (fe-np) against pc12 cell line which is known as a model of nervous system cell line was investigated by the lactate dehydrogenase (ldh) test. therefore, pc12 cells were incubated with different concentration o...

Nitric oxide (NO) is a key mediator that plays an important role in pathogenesis of various chronic diseases like Alzheimer’s disease and Parkinson’s disease. Additionally, there is a great attitude for finding natural compounds, which could control and inhibit NO production in pathological conditions. Therefore, we were encouraged to investigate the effects of some Lamiaceae species on NO prod...

Nitric oxide (NO) is a key mediator that plays an important role in pathogenesis of various chronic diseases like Alzheimer’s disease and Parkinson’s disease. Additionally, there is a great attitude for finding natural compounds, which could control and inhibit NO production in pathological conditions. Therefore, we were encouraged to investigate the effects of some Lamiaceae species on NO prod...

objective: hexavalent chromium [cr (vi)] compounds are well-known environmental contaminants  generated from industrial processes. several studies have reported the harmful effects of cr (vi) on different organs, however, little is known about neurotoxic effects of cr (vi). the aim of this study is to investigate the toxic effects of cr (vi) on pc12 cells. methods: pc12 cells were cultured foll...

Background: Aluminum (Al) exposure is among the environmental risk factors that may involve in the pathogenesis of neurodegenerative diseases. Oxidative stress has a critical role in the Al-induced toxicity. Saffron is a plant with potent radical scavenging and anti-oxidative properties. This investigation was designed to evaluate the possible protective effects of saffron extract (SE) on alumi...

Methylmercury (MeHg)-resistant sublines of rat pheochromocytoma (PC12) cells were isolated by repeated exposure to stepwise increased concentrations of MeHg. One of the sublines (PC12/TM) showed an 8- to 10-fold increase in resistance to MeHg compared with parent PC12 cells on the basis of the concentration required for 50% inhibition (IC50) of growth. PC12/TM cells accumulated smaller amounts ...