SARS-CoV-2, a single stranded positive RNA-virus, emerged in 2019 and caused global pandemic. Some infected patients develop severe complications, such as acute respiratory distress syndrome (ARDS) pulmonary fibrosis. The mechanisms leading to lung fibrosis the context of COVID-19 are still unclear. However, there correlations between this condition certain inflammatory mediators that elevated ...

We sought to determine whether the proliferation of immune cells affects the cellular uptake of the vitamin biotin. Peripheral blood mononuclear cells (PBMC) were isolated from healthy adults. The proliferation of PBMC was induced by either pokeweed lectin, concanavalin A, or phytohemagglutinin. When the medium contained a physiological concentration of [3H]biotin, nonproliferating PBMC accumul...

To elucidate the pathological roles of staphylococcal enterotoxin C (SEC) in bovine staphylococcal mastitis, a histopathological analysis of SEC-inoculated mammary glands was performed. SEC-inoculated mammary glands exhibited interstitial inflammation, and the leukocytes that migrated into the gland were predominantly polymorphonuclear neutrophils (PMN). In the gland cistern tissues dissected f...

As soon as PBMC are isolated cells start dying. The rate of apoptotic cell death increases when PBMC are shipped, cryopreserved or stored under suboptimal conditions. Apoptotic cells secrete mediators such as Lactoferrin that suppress inflammation while promoting phagocytosis. The presence of apoptotic cells in PBMC may modulate functionality in antigen-specific T cell assays such as ELISPOT or...

serotonin receptors are involved in pathophysiology of schizophrenia and may mediate other neurotransmitter effects. we investigated serotonin receptors gene expression in peripheral blood mononuclear cells (pbmc) of naïve schizophrenic patients, before and after treatment. also serotonin receptor gene expression was compared in two treatment groups including haloperidol and olanzapine. the pbm...

It is known that CD28, a positive costimulatory receptor, plays a very important role in inducing the optimal stimulation of T lymphocytes. CTLA-4 (CD152), however, acts as a negative regulator in T lymphocyte activation. The effect of an feline immunodeficiency virus (FIV) infection on the expression of feline CD28 and CTLA-4 was studied with FIV-infected and uninfected peripheral blood mononu...


 Purpose: To determine the effect of fresh soy milk and its compounds (coumestrol, daidzein genistein) on apoptosis in human leukemic cells peripheral blood mononuclear (PBMC).
 Methods: The apoptotic (K562, Jurkat, U937) PBMC was determined by flow cytometry. from healthy donors were isolated conducting density gradient centrifugation principle. Lymphoprep cytotoxicity evaluated 3-(...

In order to investigate the status of some circulating factors in nephrotic syndrome, we examined the secretion of monocyte chemotactic peptide (MCP)-1, tumor necrosis factor (TNF) alpha or fibronectin in sera or by peripheral blood mononuclear cells (PBMC) from patients with membranous nephropathy (MN), diabetic nephropathy (DN) or minimal change disease (MCD). Also the effects of PBMC or sera...

The hyper-IgD and periodic fever syndrome (HIDS) and familial Mediterranean fever (FMF) are both characterized by attacks of periodic fever accompanied by acute phase responses that are substantially higher in HIDS than in FMF. To determine whether this difference could be due to differences in production of acute phase protein-inducing mediators, we studied PBMC from HIDS and FMF patients in t...

BACKGROUND During chronic inflammation, immune cells, notably Th17 cells, infiltrate the inflammatory site and interact with local mesenchymal cells. Applied to rheumatoid arthritis (RA), the aim is to study the interactions between synoviocytes and peripheral blood mononuclear cells (PBMC) with a focus on the Th17 pathway and to identify a mechanism which leads to high IL-17 secretion with an ...