DNA reassociation was used to estimate GC content, size, and complexity of the nuclear genomes of Colletotrichum from maize and sorghum. Melting-temperature analysis indicated that the GC content of the maize pathotype DNA was 51% and that the GC content of the sorghum pathotype was 52%. DNA reassociation kinetics employing S1 nuclease digestion and an appropriately modified second-order equati...

Clubroot disease caused by Plasmodiophora brassicae is one of the most important diseases of cultivated brassicas. P. brassicae occurs in pathotypes which differ in the aggressiveness towards their Brassica host plants. To date no DNA based method to distinguish these pathotypes has been described. In 2011 polymorphism within the 28S rDNA of P. brassicae was reported which potentially could all...

the stripe (yellow) rust in wheat is one of the most important plant diseases in iran. since 1993 several epidemics have occurred in iran causing the breakdown of widely util-ized sources of resistance in wheat cultivars. twenty-seven pathotypes were identified during 2003 and 2004 in greenhouse tests. pathotypes 6e6a+, 6e22a+, 6e130a+, 6e134a+, 6e142a+, 6e158a+, 134e130a+ and 134e142a+ were mo...

INTRODUCTION Intestinal Escherichia coli pathogens are major causes of diarrhoeal disease in children under five years of age worldwide. The aim of this study is to evaluate the association of E. coli pathotypes with childhood diarrhoea in Colombia. METHODOLOGY A case-control study was conducted in 815 samples from children younger than five years of age in Cartagena, Colombia (466 cases and ...

Verticillium dahliae is a fungal pathogen that causes wilt disease in a wide range of host plants. Characterization of virulence, morphological, and molecular variations among V. dahliae isolates from different geographic origins is essential for any breeding program aimed at producing plant cultivars resistant to this disease. We characterized virulence variation among V. dahliae isolates...

Total genomic DNA from Bursaphelenchus xylophilus pathotypes MPSy-1 and VPSt-1 and from B. mucronatus was digested with restriction endonucleases. DNA fragments were electrophoretically separated, Southern blotted to nitrocellulose, and hybridized to genomic DNA from one of the isolates. The resulting hybridization patterns indicate genomic differences in repetitive DNA sequences among these po...

A 1-year prospective study was carried out in two large urban centers of São Paulo State, Brazil, to determine the prevalences and roles of the different Escherichia coli pathotypes in children less than 5 years of age with diarrhea presenting to the emergency rooms of public hospitals or visiting private pediatricians' offices. Of the pathotypes sought, typical enteroaggregative and atypical e...

Wheat stem rust, caused by Puccinia graminis f. sp. tritici ( Pgt ), has re-emerged as one of the major concerns for global wheat production since evolution Ug99 and other virulent pathotypes from East Africa, Europe, Central Asia, regions. Host resistance is most effective, economic, eco-friendly approach managing rust. Understanding virulence nature, genetic diversity, origin, distribution, e...