Attachment of relatively low numbers of endospores from two isolates of Pasteuria spp. to several species of nematodes was consistently achieved in 2-5 minutes with a centrifugation technique. The rate of attachment of Pasteuria penetrans at 10 endospores/0.1 ml/tube to second-stage juveniles (J2) of Meloidogyne javanica, M. incognita race 1, M. incognita race 3, and M. arenaria races 1 and 2 i...

This paper discusses a process of developing the data analysis and modeling Pasteuria penetrans spore attachment in vitro planta, based on observation that number spores attaching to juveniles within given time increased by increasing exposure dose. Based this, P. was modeled using negative binomial distribution which permits decomposing observation’s variability into three components: randomne...

A system for monitoring movement of the free-living second stage juveniles of root-knot nematodes (Meloidogyne spp.) using digital image analysis is described. The method is based on the analysis of video sequences of movement of individual random nematodes encumbered with or without Pasteuria penetrans endospores. Software packages were used to grab video images, to process images and to monit...

The root-knot nematode Meloidogyne incognita was controlled more effectively and yields of host plants were greater when Paecilomyces lilacinus and Pasteuria penetrans were applied together in field microplots than when either was applied alone. Yields of winter vetch from microplots inoculated with the nematode and with both organisms were not statistically different from yields from uninocula...

For many years the taxonomy of the genus Pasteuria has been marred with confusion because the bacterium could not be cultured in vitro and, therefore, descriptions were based solely on morphological, developmental, and pathological characteristics. The current study sought to devise a simple method for PCR-amplification, cloning, and sequencing of Pasteuria 16S rDNA from small numbers of endosp...

Six methods for quantification of the endospore concentrations of Pasteuria penetrans from tomato roots are described. Mortar disruption and machine disruption methods gave the highest estimations (endospores per gram of root material) of 83.7 and 79.0 million, respectively. These methods were significantly superior to incubation bioassay (47.7 million), enzymatic disruption (32.1 million), and...

Endospores of Pasteuria penetrans were evaluated for their vertical distribution in field soil and their downward movement through soil in the laboratory. In the field trial, the number of endospores attached to second-stage juveniles (J2) of Meloidogyne arenaria race 1 varied greatly in different soil depths. There were higher percentages of J2 with endospores attached in former weed fallow pl...

Pasteuria penetrans is a bacterial parasite of some plant parasite of nematodes whichcan attach to the cuticle of nematode. Differences in attachment of P. penetrans spores to the second stag juvenile (J2) cuticle may provide evidence for nematode cuticle diversity even between different ages of J2s. For investigating this purpose, we studied the effect of different ages of th...