In Paramecium, during the development of the somatic macronucleus, precise excision of thousands of single-copy non-coding sequences is initiated by specific DNA double-strand breaks, while imprecise elimination of germ-line-limited repeated sequences leads to internal deletions or chromosome fragmentation. Recent data point to a role of non-coding RNAs in the epigenetic programming of these re...

The calcium/calmodulin-regulated Ca-ATPase of the plasma membrane is implicated in Paramecium chemosensory transduction. Calmodulin antisense oligonucleotides electroporated into Paramecium disrupt chemosensory responses to sodium acetate but not to ammonium chloride.

background and objective: nitric oxide (no) plays a role in thermoregulation and growth of protozoa. this work aimed to add the molecule no in physiology of protozoa in contact with abused narcotic substances. materials and methods: a sedative drug, morphine, was infused into a cell chamber containing paramecia . the cell response to the drug was recorded promptly after drug infusion using a po...

introduction: response to morphine and role of nitric oxide (no) on expression of morphine response has been studied in vertebrates. but, little evidence is provided in the matter in earlier invertebrates. this investigation for the first time evaluated the effect of no on expression of morphine potency in paramecium caudatum. methods: animal after isolation from natural media and specific iden...

1 Graham, R. M., Surg. Gynec. & Obst., 84, 153-173 (1947). 2 Henshaw, P. S., J. Nat. Cancer Inst., 4, 485-501 (1944). 3Larkin, J. C., Am. J. Roentgenol., 55, 525-532 (1946). 4 Hektoen, L., J. Infect. Dis., 22, 28-33 (1918). 6 Auer, J., and Witherbee, W. D., J. Exper. Med., 33, 791-813 (1921). 6 Treadwell, H., Gardner, W. V., and Laurence, J. H., Endocrinology, 32, 161-164 (1943). 7 EIlinger, F....

Caedibacter taeniospiralis is an obligate endosymbiont living in the cytoplasm of Paramecium tetraureliaC. taeniospiralis causes the so-called killer trait, eliminating intraspecific competitors of its host when released into the medium by the concerted action of the unusual protein structure R-body (refractile body) in addition to an as-yet-unknown toxin.

1. The physiological actions of HCN and its salts appear to be due (a) to the ease with which HCN molecules penetrate living cells and then ionizing, exert their influence by means of H ions and CN ions; (b) to the weakness of HCN as an acid, which permits at neutrality or at even slight alkalinity the presence of a considerable amount of free HCN molecules in the presence of their salts; (c) t...

The mitochondrial (mt) encoded ndh1, ndh3, ndh4, ndh5, rpl14, cyt b and atp9 gene products were identified by sequence comparisons with known proteins. Amino acid sequence comparisons between predicted Paramecium mt gene products and proteins in current databases were quantitated approximately by the means of similarity scores for pairs of aligned sequences. The comparisons show that the Parame...

Modelling and analysis are presented for excitation of paramecium. Excitations are characterized by difference in time durations (pulse and plateau) and polarities (positive and negative) of potentials. The former is used for backward swimming against stimulus at the front of the body, the latter is used fir forward fast swimming against stimulus at rear of the body. This paper gives electro-ph...

Polyclonal (PAT) and monoclonal (AXO 49) antibodies against Paramecium axonemal tubulin were used as probes to reveal tubulin heterogeneity. The location, the nature and the subcellular distribution of the epitopes recognized by these antibodies were, respectively, determined by means of: (i) immunoblotting on peptide maps of Paramecium, sea urchin and quail axonemal tubulins; (ii) immunoblotti...