نتایج جستجو برای: page protein analysis
تعداد نتایج: 3877308 فیلتر نتایج به سال:
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Background: Rabies virus (RABV) is a deadly neurotropic virus that causes the disease of rabies in humans and animals. L protein is one of the large structural protein of rabies virus, which displays multiple enzymatic activities, and is required for viral transcription and replication. Objectives: A truncated L protein of Rabies virus is being cloned, expressed and purified to produce relevant...
Analysis of transgenic plants is very important in gene transfer programs. In this research, the second generation (T1) of transgenic brassica napus which was transformed by antisense of Glutamine synthetase (GS) gene was studied from the view of total soluble protein content of leaf, total chlorophyll and protein patterns (SDS-PAGE) using seeds of Brassica napus .Protein concentration was dete...
Analysis of transgenic plants is very important in gene transfer programs. In this research, the second generation (T1) of transgenic brassica napus which was transformed by antisense of Glutamine synthetase (GS) gene was studied from the view of total soluble protein content of leaf, total chlorophyll and protein patterns (SDS-PAGE) using seeds of Brassica napus .Protein concentration was dete...
Brucellosis is caused by the bacterium Brucella and affects various domestic and wild species. GroEL (Heat Shock Protein 60kDa) as one of the major antigens that stimulate the immune system, increases Brucella survival. The aim of the current study was to clone and express GroEL in Escherichia coli in order to design subunit vaccine. Amplifying was performed using specific primers. The full-len...
Background Human coagulation factor IX is a 57kDa plasma serine protease made in Liver which plays a vital role in the blood coagulation cascade. FIX deficiency causes severe disorder Hemophilia B or Christmas disease. Nowadays, recombinant proteins have important roles in treatment of diseases. Although, cultivated mammalian cells because of their ability for producing properly folded protein ...
conclusions in this study, the optimized truncated orf2 protein was expressed in e. coli successfully and the highly purified protein was obtained, which can be a potential vaccine candidate and as an antigen in elisa to diagnose hev infections. results the subcloning was confirmed by pcr, restriction enzyme digestion, and dna sequencing of recombinant plasmid pet30a-orf2.2. the results obtaine...
we have modified one of the most useful methods of protein separation; namely, two dimensional gel electrophoresis (2-de). this modified version of 2-de is not only simpler and easier but also faster than all the currently available methods. in this method, isoelectric focusing is carried out in the first dimension using a vertical sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-...
background: rh (rhesus) is a highly complex blood group system in man deeply rooted in transfusion medicine. isolation of rhd from cord blod, cloning and expression of recombinant rhd antigen in bacterial expression system was the aim of this study. methods: total rnas were extracted from cord blood (o + ). the quality of rna was determined by electrophoresis. in order to obtain coding sequen...
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