Ghrelin is a regulatory peptide with specific anti-oxidative effects. The aim of this study was to investigate the activity of two antioxidant enzymes includes superoxide dismutase (SOD) and glutathione peroxidase (GPx) in erythrocyte of newly-hatched chicken following in ovo administration of ghrelin. The fertilized eggs were divided into four experimental groups; control G1 (without injection...

The objective of this study was to test the hypothesis that in ovo injection of carbohydrates into pigeon (Columba livia) amnion may improve the small intestine development. At d 14.5 of incubation, 80 fertile eggs were injected with 200 μL of carbohydrate solution, and 80 control eggs were not injected. The carbohydrate solution (wt/vol) contained 2.5% maltose + 2.5% sucrose, all dissolved in ...

The effects of the in ovo injection of commercial diluent containing various levels of 25-hydroxycholecalciferol [25(OH)D3] on hatchability and hatching chick quality variables in Ross × Ross 708 broilers were examined in 2 trials. All treatment groups, each containing 21 and 40 eggs in trials 1 and 2, respectively, were randomly represented on each of 10 replicate tray levels of a single-stage...

this study was conducted to evaluate the long-term effects of in ovo injection of nano-silver, thyme and savory extracts in broiler breeder eggs on growth performance, lymphoid organ weights, immune and blood parameters and bone mineral concentration of broiler chicks. a total of 560 fertilized broiler breeder eggs were divided into 7 groups. on d 5 of incubation, eggs were treated with the fol...

This study was conducted to evaluate the long-term effects of in ovo injection of nano-silver, thyme and savory extracts in broiler breeder eggs on growth performance, lymphoid organ weights, immune and blood parameters and bone mineral concentration of broiler chicks. A total of 560 fertilized broiler breeder eggs were divided into 7 groups. On d 5 of incubation, eggs were treated with the fol...

in this research 400 ross 308 breeder fertile eggs were weighed at day 16 of incubation and evenly distributed into 4 treatments with 4 replicates per treatment and 25 eggs per replicate in a crd design. on day 18 of incubation, 1 ml of an in ovo feed solution including: i) carbohydrate (cho), ii) amino acid (aa), and iii) butyric acid solution in 0.5% saline was injected into amniotic fluid of...

INTRODUCTIONIn ovo RNA interference (RNAi) is a method for silencing a gene of interest using a combination of in ovo injection and electroporation in avian embryos. Here we describe gene silencing in the developing spinal cord, but the procedure can easily be adapted to other parts of the nervous system. Double-stranded RNA (dsRNA) derived from the gene of interest is injected into the develop...

Non-invasive assessment of the biodistribution is of great importance during the development of new pharmaceutical compounds. In this contribution, the applicability of in ovo MRI for monitoring the biodistribution of MR contrast agent-labelled compounds was investigated in mamaria carcinomas xentotransplanted on the chorioallantoic membrane (CAM) exemplarily for Gd-DOTA and cHSA-PEO (2000)16-G...