Cryopreservation of embryos and gametes has become an indispensible service for infertility treatment. Vitrification is now a prevalent technique for cryopreservation in assisted reproductive technology (ART) replacing, in many cases, conventional slow cooling methodology. Fertility preservation in female patients has become a very topical issue. Various malignant and nonmalignant diseases have...

objective: this study aimed to evaluate the incidence of apoptosis in vitrified and non-vitrified human ovarian tissue by the use of morphological analysis and apoptosis assay techniques. methods: we obtained human ovarian tissue biopsies from 30 women who underwent elective caesarean sections. tissues were transported to the laboratory in pre-warmed, equilibrated leibovitz l-15 medium within 2...

in recent years, preservation of fertility in women has been of great importance, especially in patients exposed to deleterious conditions on fertility. thus, cryopre-servation of human gametes, embryos and ovarian tissue has become an essential part of assisted reproduction. this approach limits the number of embryos transferred, while supernumerary oocytes and/ or embryos can be used in subse...

Background In vitro culturing and retransplantion of vitrified- warmed ovarian tissue are two ways to restore fertility after radiation or chemotherapy.This study aimed to evaluate the incidence of apoptosis in vitrified human ovarian tissue after warming, long term culturing and xenotransplantation by morphological analyses and apoptosis genes expression evaluation. MaterialsAndMethods We obta...

introduction: this study was performed to investigate the effect of vitrification procedure using ethyleneglycol as cryoprotectant on the follicular morphology of neonatal ovarian tissue of mouse after thawing andautotransplantation. material and methods: ovaries from 3 weeks neonate nmri mice were excised then vitrified in asolution of dmem medium containing ficol 70, sucrose, acetanied and et...

Background: Mesenchymal stem cells (MSCs) can be used to treat premature ovarian failure (POF). Different methods have already been applied to detect MSCs in tissues. This study aimed to investigate the quantitative distribution of CM-DiI-labeled human umbilical cord vein MSCs (hUCV-MSCs) in different regions of the ovarian tissue of the cyclophosphamide ( CTX )-induced POF in mice. Methods: Ad...

Background Ovarian tissue cryopreservation represents a promising strategy to preserve the ovarian function in cancer patients. It is usually performed by slow freezing/rapid thawing (SF/RT). Recent studies emphasize an ultrarapid cryopreservation procedure, vitrification/warming (V/W), since it might prevent damages due to ice crystal formation. Comparative studies between the cryopreservation...

Background Ovarian tissue cryopreservation represents a promising strategy to preserve the ovarian function in cancer patients. It is usually performed by slow freezing/rapid thawing (SF/RT). Recent studies emphasize an ultrarapid cryopreservation procedure, vitrification/warming (V/W), since it might prevent damages due to ice crystal formation. Comparative studies between the cryopreservation...

Background. Hormone disorders, stress-oxidative and inflammation in ovarian tissue cause no- ovulation in women with polycyclic ovarian syndrome (PCOS). Several studies have shown the role of exercises in control of sex hormones. Objectives. The aim of this study was to investigate the effect of 6 weeks of swimming training (ST) on serum levels of sex hormones, antioxidant enzymes and oxidativ...