Previous studies have reported many discrepancies about the best type and concentration of cryoprotective agents (CPAs) and biological variability among various pre-antral follicle classes after cryopreservation of ovarian tissue. The aim of this study was to investigate the impacts of some synthetic polymers on histological characteristics of different types of pre-antral follicles after bovin...

1. Abstract 2. Introduction 3. Apoptosis during follicular development 3.1. Follicular development 3.2. Follicular atresia 4. Hormonal regulation of ovarian apotosis 4.1. Primordial follicles 4.2. Preantral follicles 4.3. Early antral follicles 4.4. Preovulatory follicles 4.5. Periovulatory follicles 5. Apoptosis in corpus luteum 6. Signal pathway of factors in regulating apoptosis in ovary 6.1...

Background The aim of this study was to investigate the effect of applying the 1-mT magnetic field (MF) in the ovarian tissue vitrification. MaterialsAndMethods Ovaries of 6-8 week-old Naval Medical Research Institute female mice randomly were divided into 5 groups: V1: Ovaries that immediately after leaving of the mice body, were examined histologically (control group), V2: Ovaries that withou...

Introduction: Genital system is important herd in animal's creation. It is necessary for animal reproduction. Mammal has two sex in genital system such as male and female in separated animals. Animal reproduction destroyed by disorder of genital system. In this case, animals cannot produce an animal similar to them. Finally it cause overthrow a species of animals. Methods: Histomorpholog...

A large proportion of follicles are lost during the initial ischemia that occurs after transplantation of ovarian tissues. Thus, the effect of hyperbaric oxygen therapy (HBO) on the follicular loss of ovarian tissues after transplantation was examined in mice. Ovarian slices from ICR mice were transplanted under the kidney capsule in ovariectomized ICR. Hyperbaric oxygen with 100% oxygen was in...

BACKGROUND New data suggests that endothelial cells (ECs) elaborate essential "angiocrine factors". The aim of this study is to investigate the role of activated ovarian endothelial cells in early in-vitro follicular development. METHODS Mouse ovarian ECs were isolated using magnetic cell sorting or by FACS and cultured in serum free media. After a constitutive activation of the Akt pathway w...

BACKGROUND Premature ovarian failure (POF) is generally irreversible. However, developing follicles up to the antral stage are reported in POF and anti-Müllerian hormone (AMH) might be a good indicator of follicular presence. This study analysed serum AMH, ovarian histology and AMH immunoexpression in POF patients. METHODS A cross-sectional study of 48 POF patients in an Endocrinology Departm...

The objective of this study was to evaluate the effects of adding ascorbic acid to the media for in vitro culture of cattle ovarian fragments and to determine their effects on growth activation and viability of early-stage follicles. The ovarian cortex was divided into small fragments; one fragment was immediately fixed (control) and the other fragments were cultured in minimum essential medium...

BACKGROUND The stripping technique for endometriomas excision has been reported to be associated with follicular loss. The objective of this trial was to evaluate the presence and nature of ovarian tissue adjacent to the endometrioma cyst wall obtained by stripping with different techniques. METHODS Forty-eight patients with ovarian endometrioma were enrolled in two consecutive independent ra...

BACKGROUND Ovarian tissue cryopreservation by vitrification is an attractive technique for fertility preservation in women. However, this technique has not been optimized. The aim of this study was to evaluate the baboon as a model for the preclinical study of ovarian tissue cryopreservation by vitrification and thawing. METHODS Ovarian cortical tissues (1-mm cubes) were obtained surgically f...