The successful classification of Group A streptococci by the capillary precipitin technique requires a complete series of M type antisera which are sufficiently potent and specific to give unequivocal type-specific reactions with all the serotypes. Specific antisera for this purpose have been prepared by absorption with heterologous streptococci. Unabsorbed antisera have been employed here in t...

As a fundamental experiment for the study on the role of growth hormone in mouse mammary gland, some immunological properties of rabbit antiserum to mouse growth hormone (MGH) were studied. Antiserum was obtained by immunizing New Zealand White male rabbits with MGH preparation extracted from mouse anterior pituitaries. The precipitin titer of the antiserum by precipitin ring test against MGH p...

Crosslinking of ultrapure hemoglobin, crystalline catalase, and superoxide dismutase resulted in a soluble nanodimensional complex of polyhemoglobin-catalase-superoxide dismutase. A less expensive and more convenient way is to crosslink bovine stroma-free hemolysate (stroma-free hemolysate) that already contains hemoglobin, catalase, and superoxide dismutase into polyhemoglobin with catalase an...

Cholera enterotoxin (CT) and heat-labile enterotoxin (LT) produced by enterotoxigenic Escherichia coli share a common antigenic determinant. In addition, CT and LT each have a unique antigenic determinant. Antisera were prepared by immunoaffinity chromatography against these unique antigenic determinants, that is, antiserum that reacted with CT but not with LT and antiserum that reacted with LT...

Immunologic cross-reactivity between Streptococcus pyogenes type A exotoxin (erythrogenic toxin) and Staphylococcus aureus enterotoxins B and C1 was demonstrated by Ouchterlony double diffusion, Western immunoblot, and immunodot analyses. Specific antiserum to type A streptococcal exotoxin reacted more strongly with staphylococcal enterotoxin B than with enterotoxin C1. The reactivity of type A...

Antisera to mucin of benign mucinous cystadenomas of human ovary were produced in rabbits. These antisera were reacted with cancerous and normal tissue extracts by the Ouchterlony double diffusion test. Distinctive precipitin reactions occurred with colon cancer mucosal extracts. These bands were distinctive for colon cancer and could not be removed by absorption with normal colon mucosa from t...

Parent bacteria and L-forms of some streptococci of group A, group D, and Streptococcus MG share common antigens as shown by cross-reactivity in growth inhibition, complement fixation, and Ouchterlony tests. Cross-reactivity between these streptococci or their L-forms with a staphylococcal L-form, or Mycoplasma pneumoniae, was found only rarely and only in low-titer complement fixation tests. R...

Rabbit antisera to newt (T. cristatus) globin were produced by repeated injections of globin and antiglobin antibodies purified by chromatography on globin-Sepharose 4B. Ouchterlony and SDS PAGE analysis indicated that the material eluted from the affinity column was rabbit IgG. The antiglobin antibodies tested by immunodiffusion and ELISA cross-reacted with native hemoglobin and globin from T....

A practical immunologic assay to detect the hormone, prolactin, in body fluids was evaluated. Prolactin content was assayed using positive antiprolactin sera prepared in rabbits and test samples of serum, urine, and urinary extract from a borane exposed female monkey in micro-Ouchterlony gel diffusion and agglutination inhibition tests. The gel diffusion test gave qualitative results while the ...