نتایج جستجو برای: orf2 protein

تعداد نتایج: 1234968  

Journal: :Journal of Biomedicine and Biotechnology 2001
Li Xiaofang Mohammad Zafrullah Faizan Ahmad Shahid Jameel

Hepatitis E virus (HEV) is the causative agent of hepatitis E, an acute form of viral hepatitis. The open reading frame 2 (ORF2) of HEV encodes the viral capsid protein, which can self-oligomerize into virus-like particles. To understand the domains within this protein important for capsid biogenesis, we have carried out in vitro analyses of association and folding patterns of wild type and mut...

Journal: :Journal of virology 2013
Daraporn Pittayakhajonwut Devis Sinani Clinton Jones

Bovine herpesvirus 1 (BHV-1), like other members of the Alphaherpesvirinae subfamily, establishes latency in sensory neurons. The virally encoded latency-related RNA (LR-RNA) is expressed abundantly in latently infected sensory neurons and encodes several proteins, including ORF2. An LR mutant virus with stop codons at the amino terminus of ORF2 does not reactivate from latency after treatment ...

Journal: :Journal of bacteriology 2013
Neta Sal-Man Dheva Setiaputra Roland Scholz Wanyin Deng Angel C Y Yu Natalie C J Strynadka B Brett Finlay

Type III secretion systems (T3SSs) are central virulence mechanisms used by a variety of Gram-negative bacteria to inject effector proteins into host cells. The needle polymer is an essential part of the T3SS that provides the effector proteins a continuous channel into the host cytoplasm. It has been shown for a few T3SSs that two chaperones stabilize the needle protein within the bacterial cy...

2015
Fatemeh Farshadpour Manoochehr Makvandi Reza Taherkhani

BACKGROUND Hepatitis E Virus (HEV) is the causative agent of enterically transmitted acute hepatitis and has high mortality rate of up to 30% among pregnant women. Therefore, development of a novel vaccine is a desirable goal. OBJECTIVES The aim of this study was to construct tPAsp-PADRE-truncated open reading frame 2 (ORF2) and truncated ORF2 DNA plasmid, which can assist future studies with...

2009
Xiu-Fang Wang Xia Jin Xiaoyan Wang Jing Liu Jingjing Feng QinQing Yang Wenli Mu Xiaojuan Shi Zhanjun Lu

The retrotransposon known as long interspersed nuclear element-1 (L1) is 6 kb long, although most L1s in mammalian and other eukaryotic cells are truncated. L1 contains two open reading frames, ORF1 and ORF2, that code for an RNA-binding protein and a protein with endonuclease and reverse transcriptase activities, respectively. In this work, we examined the effects of full length L1-ORF2 and OR...

Journal: :The Journal of veterinary medical science 2004
Jung-Yeon Kim Naoaki Yokoyama Sanjay Kumar Noboru Inoue Kozo Fujisaki Chihiro Sugimoto

In the present study, a novel antigenic protein expressed in the piroplasm stage of Theileria orientalis was characterized. A 4,707 bp genomic fragment amplified by PCR contained two open reading frames (ORFs). The deduced amino acid sequence of the first ORF showed significantly high similarlity to the ubiquitin carboxy terminal hydrolases/proteases while the second ORF (To ORF2) showed homolo...

2015
Ying Qi Feng Zhang Li Zhang Tim J. Harrison Weijin Huang Chenyan Zhao Wei Kong Chunlai Jiang Youchun Wang Xiang-Jin Meng

The absence of a productive cell culture system hampered detailed analysis of the structure and protein composition of the hepatitis E virion. In this study, hepatitis E virus from a robust HEV cell culture system and from the feces of infected monkeys at the peak of virus excretion was purified by ultra-centrifugation. The common feature of the two samples after ultracentrifugation was that th...

Journal: :Journal of virology 1999
A de Parseval J H Elder

The long PCR technique was used to amplify the three size classes of viral mRNAs produced in cells infected by feline immunodeficiency virus (FIV). We identified in the env region a new splice acceptor site that generated two transcripts, each coding for an 11-kDa protein, p11(rev), whose function is unknown. The small-size class of mRNAs included two bicistronic orf2/rev mRNAs and two rev-like...

2015
Dong-hai Peng Cui-yun Pang Han Wu Qiong Huang Jin-shui Zheng Ming Sun

The insecticidal crystal protein (Cry) genes of Bacillus thuringiensis are a key gene resource for generating transgenic crops with pest resistance. However, many cry genes cannot be expressed or form crystals in mother cells. Here, we report a novel Cry protein gene, cry65Aa1, which exists in an operon that contains a downstream gene encoding a hypothetical protein ORF2. We demonstrated that O...

2014
Yoshinori Hayashi Masaki Kajikawa Takuma Matsumoto Norihiro Okada

LINEs mobilize their own copies via retrotransposition. LINEs can be divided into two types. One is a stringent type, which constitutes a majority of LINEs. The other is a relaxed type. To elucidate the molecular mechanism of retrotransposition, we used here two different zebrafish LINEs belonging to the stringent type. By using retrotransposition assays, we demonstrated that proteins (ORF2) en...

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