Purine metabolism is known factor for nuclear maturation of oocytes through both follicle cells and oocyte itself. However, it is largely unknown the roles of purine metabolism in the oocyte competence for fertilization and early development. In this study, the effects of adenosine in oocyte competence for development were examined using adenosine and its synthetic inhibitors. Adenosine treatme...

conclusions the present study showed that vitrification using cryotop and freezing medium can damage oocytes by reducing the maturation and fertilization rates in both developmental stages. results vitrification caused a significant reduction in the maturation rate of oocytes. of those that matured, the fertilization rate of vitrified ivm-mii (44.1%) and ov-mii oocytes (50%) was not significant...

Background: The cryopreservation of human oocyte would make significant contribution to fertility treatments. It could also provide on alternative to embryo preservation for avoidance ethical problems. Embryo cryopreservation is now a successful procedure, but oocyte cryopreservation has poorer results. Materials and Methods: For retrieval of oocyte NMRI mouse were used. after administration of...

OBJECTIVE To investigate the association of individual follicular fluid (FF) leptin and adiponectin levels with the quality of the corresponding oocyte and embryo. METHODS We prospectively enrolled 67 women who underwent controlled ovarian hyperstimulation with 89 FF samples. FF and the corresponding oocyte was obtained from a single dominant preovulatory follicle at the time of oocyte retrie...

background: in vitro maturation (ivm) of oocytes is a promising technique to reduce the costs and avert the side-effects of gonadotropin stimulation for in vitro fertilization (ivf). the pregnancy rates from oocytes matured in vitro are much lower than those of in vivo stimulation cycles, indicating that optimization of ivm remains a challenge. objective: in this study, we investigated the effe...

Fertilization marks the end of oogenesis and the beginning of embryogenesis. The legacy of oogenesis is, however, maintained by virtue of the stored nutritional and regulatory molecules that fuel, and in many cases direct, early development. The signal that stimulates the transition from oocyte to embryo is a sperm-induced increase in the concentration of intracellular free Ca2+. Thus successfu...

Fertilization involves cell-cell fusion of a sperm with the oocyte. This fusion restores the diploid genome, activates the oocyte, and initiates embryonic development. The identification of proteins mediating the fusion of sperm with oocyte plasma membrane (oolemma) is important to a deeper knowledge of fertilization. Defects in sperm-oocyte fusion may account for some form of human infertility...

Low and/or total failed fertilization following ICSI is a disappointing effect that some couples may face during their treatment which may have severe social and economic consequences for these infertile couples. This phenomenon has been mainly attributed to failed oocyte activation post ICSI. Researchers have regarded this failure due to the absence or deficiency of sperm associated to oocyte ...

OBJECTIVE To compare the outcomes of embryo development between oval-shaped oocyte as an abnormal morphology oocyte and a normal morphology oocyte in In Vitro Fertilization (IVF). METHODS This study was a comparative analytical study with retrospective approach which lasted from September 2014 until November 2015. For this study, we used secondary data (medical records) from 24 patients submi...

Improved methods are needed to reliably and accurately evaluate oocyte quality prior to fertilization and transfer into the woman of human embryos created through in vitro fertilization (IVF). All oocytes that are retrieved and matured in culture are exposed to sperm with little in the way of evaluating the oocyte quality. Furthermore, embryos created through IVF are currently evaluated for dev...