The nucleus is the most prominent compartment of any eukaryotic cell and home to its genetic information. The nucleoplasm is surrounded by a double membrane system, the nuclear envelope (NE). The outer nuclear membrane (ONM) and the inner nuclear membrane (INM) are separated by the perinuclear space (or periplasmic space; Hetzer et al., 2005). The lipid bilayer of the ONM is continuous with the...

Inner nuclear membrane Sad1/UNC-84 (SUN) proteins interact with outer nuclear membrane (ONM) Klarsicht/ANC-1/Syne homology (KASH) proteins, forming linkers of nucleoskeleton to cytoskeleton conserved from yeast to human and involved in positioning of nuclei and chromosomes. Defects in SUN-KASH bridges are linked to muscular dystrophy, progeria, and cancer. SUN proteins were recently identified ...

Background. There is a growing controversy about the use of oronasal masks (ONM) or total facemask (TFM) in noninvasive positive pressure ventilation (NPPV), so we designed a trial to compare the uses of these two masks in terms of effectiveness and comfort. Methods. Between February and November 2014, a total of 48 patients with respiratory failure were studied. Patients were randomized to rec...

Despite their importance in cell biology, the mechanisms that maintain the nucleus in its proper position in the cell are not well understood. This is primarily the result of an incomplete knowledge of the proteins in the outer nuclear membrane (ONM) that are able to associate with the different cytoskeletal systems. Two related ONM proteins, nuclear envelope spectrin repeat (nesprin)-1 and -2,...

Klarsicht/ANC-1/Syne/homology (KASH)/Sad-1/UNC-84 (SUN) protein pairs can act as connectors between cytoplasmic organelles and the nucleoskeleton. Caenorhabditis elegans ZYG-12 and SUN-1 are essential for centrosome-nucleus attachment. Although SUN-1 has a canonical SUN domain, ZYG-12 has a divergent KASH domain. Here, we establish that the ZYG-12 mini KASH domain is functional and, in combinat...

Let M be a smooth manifold of positive dimension n equipped with a smooth density dμM. Let A be a polyhomogeneous elliptic pseudo-differential operator of positive order m onM which is symmetric for the L scalar product defined by dμM. For each L > 0, the space UL = ⊕ λ≤LKer(A− λId) is a finite dimensional subspace of C∞(M). Let ΠL be the spectral projector onto UL. Given s ∈ R, we compute the ...

Nuclear pore complexes (NPCs) assemble at the end of mitosis during nuclear envelope (NE) reformation and into an intact NE as cells progress through interphase. Although recent studies have shown that NPC formation occurs by two different molecular mechanisms at two distinct cell cycle stages, little is known about the molecular players that mediate the fusion of the outer and inner nuclear me...