Modified 8–17 DNAzymes that can induce target gene silencing in cells were developed based on the X-ray crystal structure of DNAzyme and modifications used designing antisense oligonucleotides.

Cyc1 mutants of the yeast Saccharomyces cerevisiae were directly transformed with both sense and antisense oligonucleotides to examine the involvement of the two genomic DNA strands in transformation. Sense oligonucleotides yielded approximately 20-fold more transformants than antisense oligonucleotides. This differential effect was observed with oligonucleotides designed to make alterations at...

Oligonucleotides containing 1-(2,4-dideoxy-beta-D-erythro-hexopyranosyl)thymine (2) and 1-(3,4-dideoxy-beta-D-erythro-hexopyranosyl)thymine (3) were synthesized on a solid support using the phosphoramidite approach. The properties of these oligonucleotides were compared with the earlier reported characteristics of oligonucleotides containing 1-(2,3-dideoxy-beta-D-erythro-hexopyranosyl) thymine ...

Molecular imaging, the science that combines non-invasive in vivo imaging and molecular biology, has begun to use labelled oligonucleotides as radiotracers. Antisense oligonucleotides target gene expression at the RNA level, while aptamer oligonucleotides are designed to hit proteins of interest. Oligonucleotides for imaging cover a large range of applications, from the invention of new contras...

This work describes the application of a pressure-enhanced liquid chromatography (PE-LC) setup to tune separation various large molecules comprised nucleic acids (oligonucleotides, messenger ribonucleic acid [mRNA], and deoxyribonucleic [DNA]). When adding pressure as method development parameter, it indeed becomes possible modify retention, selectivity, peak width. As an example, oligonucleoti...

ABSTRACT INTRODUCTION: The human papillomavirus (HPV) detection favors treatments for patients with clinical manifestations and limits future consequences those asymptomatic infections. OBJECTIVES: Therefore, the present study aimed to evaluate sensitivity of polymerase chain reaction (PCR) HPV from oral mucosa samples, laryngeal papillomatosis. MATERIAL AND METHODS: A total 49 pediatric patien...

A novel 2'-modification, 2'-O-[2-(methylthio)ethyl] or 2'-O-MTE, has been incorporated into oligonucleotides and evaluated for properties relevant to antisense activity. The results were compared with the previously characterized 2'-O-[2-(methoxy)ethyl] 2'-O-MOE modification. As expected, the 2'-O-MTE modified oligonucleotides exhibited improved binding to human serum albumin compared to the 2'...