Bis zu einem Viertel der RA-Patienten sprechen nicht auf biologische und zielgerichtete synthetische krankheitsmodifizierende Antirheumatika (b/tsDMARDs) an benötigen weitere Behandlungsversuche. Ob diese Patienten von wiederholten Versuchen mit anderen b/tsDMARDs profitieren, ist unbekannt. Zhao et al. beschrieben Behandlungsergebnisse aufeinanderfolgenden Therapielinien bei RA-Patienten, die ...

Glucocorticoid therapy, especially at higher doses, is associated with significant adverse side effects including osteoporosis. Leptin, secreted from adipose tissue, has diverse effects on bone tissue regulation. As glucocorticoids stimulate leptin synthesis and secretion directly in adipose tissue we hypothesised that dexamethasone (DEX) induced osteoporosis may, in part, be mediated by an ost...

BACKGOUND The formation of bony spurs and ankylosis is a key pathognomic feature in ankylosing spondylitis (AS) and results in functional impairment. The aim of this study was to investigate the role of IL-32γ in osteoblast (OB) differentiation and its association with the pathogenesis of AS. METHODS The concentration and expression of IL-32γ were evaluated in synovial fluid and tissue from p...

Leptin or obesity receptor (Ob-R) is a member of class I cytokine receptor family. Ob-R, expressed in six isoforms, is the product of alternative RNA splicing of db gene. According to its structural differences, the receptor's isoforms are divided into three classes: long, short, and secretory isoforms. A long, fully active isoform of Ob-Rb is expressed mainly in the hypothalamus, where it take...

Because leptin has recently been shown to induce proliferation and/or differentiation of different cell types through different pathways, the aim of the present study was to investigate, in vitro, the influence of leptin on adipogenesis in rat preadipocytes. A prerequisite to this study was to identify leptin receptors (Ob-Ra and Ob-Rb) in preadipocytes from femoral subcutaneous fat. We observe...

We numerically analyze Non-Oberbeck-Boussinesq (NOB) effects in two-dimensional Rayleigh-Bénard flow in glycerol, which shows a dramatic change in the viscosity with temperature. The results are presented both as functions of the Rayleigh number Ra up to 10 (for fixed temperature difference ∆ between the top and bottom plates) and as functions of ∆ (“nonOberbeck-Boussinesqness” or “NOBness”) up...

Reduced leptin (Ob protein) signaling is proposed to be a stimulus for the activation of neuropeptide Y (NPY) gene activity and increased expression of mRNA for the long form of the leptin receptor (Ob-Rb) in the hypothalamic arcuate nucleus. To determine if Ob-Rb protein is expressed in arcuate nucleus NPY neurons, we developed an affinity-purified polyclonal antibody against amino acids 956-1...

Leptin deficiency causes obesity in rodents and humans, but circulating levels of leptin are paradoxically elevated in obesity. The mechanisms underlying this leptin resistance are unknown, but may involve reduced leptin transport across the blood-brain barrier via short isoforms of the leptin receptor (Ob-R). Here, we first quantified short Ob-R mRNA expression in isolated rat cerebral microve...