Reactive oxygen species (ROS) play a divergent role in both cell survival and cell death during ischemia/reperfusion (I/R) injury and associated inflammation. In this study, ROS generation by activated macrophages evoked an intracellular Ca2+ ([Ca2+]i) transient in endothelial cells that was ablated by a combination of superoxide dismutase and an anion channel blocker. [Ca2+]i store depletion, ...

Experiments were conducted to determine the respiration rate of tomato at 10, 20 and 30 °C using closed respiration system. Oxygen depletion and carbon dioxide accumulation in the system containing tomato was monitored. Respiration rate was found to decrease with increasing CO2 and decreasing O2 concentration. Michaelis-Menten type model based on enzyme kinetics was evaluated using experimental...

Reactive oxygen species (ROS) have been implicated in both cell signaling and pathology. A major source of ROS in endothelial cells is NADPH oxidase, which generates superoxide (O2 ) on the extracellular side of the plasma membrane but can result in intracellular signaling. To study possible transmembrane flux of O2 , pulmonary microvascular endothelial cells were preloaded with the O2 -sensiti...

PX-12, as an inhibitor of thioredoxin (Trx), has antitumor activity. However, little is known about the toxicological effect of PX-12 on cervical cancer cells. In the present study, the growth inhibitory effects of PX-12 on HeLa cervical cancer cells in association with reactive oxygen species (ROS) and glutathione (GSH) levels were investigated. Based on MTT assays, PX-12 inhibited the growth ...

Growing interest in the development of a hydrogen economy means that CO oxidation is increasingly important for upgrading H2-rich fuel gas streams cells. CeO2-supported catalysts are most promising candidates catalytic because their high activity. In present work, DFT+U calculations were performed to investigate stability and reactivity Ptn (n = 1?4) clusters supported on CeO2(111) (Pt/CeO2) Pt...

Pulmonary emphysema impairs lung and respiratory muscle function leading to restricted physical capacity and accelerated morbidity and mortality consequent to respiratory muscle failure. In the absence of direct evidence, an O2 supply-demand imbalance within the diaphragm and other respiratory muscles in emphysema has been considered the most likely explanation for this failure. To test this hy...

Nazarewicz RR, Dikalova AE, Bikineyeva A, Dikalov SI. Nox2 as a potential target of mitochondrial superoxide and its role in endothelial oxidative stress. Am J Physiol Heart Circ Physiol 305: H1131–H1140, 2013. First published August 16, 2013; doi:10.1152/ajpheart.00063.2013.—Superoxide (O2 ) production by the NADPH oxidases is implicated in the pathogenesis of many cardiovascular diseases, inc...

We reconstructed oxygenation changes in the upwelling ecosystem off Concepción (36 S), Chile, using inorganic and organic proxies in a sediment core covering the last ca. 110 years of sedimentation in this area. Authigenic enrichments of Mo, U and Cd were observed between ca. 1935 and 1971 CE, implying a prolonged period with predominantly more reduced conditions in bottom waters and surface se...

A metal-accumulating willow was grown under greenhouse conditions on a Zn/Cd-polluted soil to investigate the effects of sulfur (S(0)) application on metal solubility and plant uptake. Soil porewater samples were analyzed 8 times during 61 days of growth, while DGT-measured metal flux and O2 were chemically mapped at selected times. Sulfur oxidation resulted in soil acidification and related mo...