Apaf-1(-/-) or caspase-3(-/-) cells treated with a variety of apoptosis inducers manifest apoptosis-associated alterations including the translocation of apoptosis-inducing factor (AIF) from mitochondria to nuclei, large scale DNA fragmentation, and initial chromatin condensation (stage I). However, when compared with normal control cells, Apaf-1(-/-) or caspase-3(-/-) cells fail to exhibit oli...

Recent studies indicate that controlling the nuclear decondensation and intra-nuclear localization of plasmid DNA (pDNA) would result in an increased transfection efficiency. In the present study, we established a technology for imaging the nuclear condensation/decondensation status of pDNA in nuclear subdomains using fluorescence resonance energy transfer (FRET) between quantum dot (QD)-labele...

The onset of quartetting, i.e. α-particle condensation, in symmetric nuclear matter is studied with the help of an in-medium modified four nucleon equation. It is found that at very low density quartetting wins over pairing, because of the strong binding of the α-particles. The critical temperature can reach values up to around 6 MeV. Also the disappearance of α-particles with increasing densit...

The present study was undertaken to provide complementary data on the heterochromatin condensation in both central and peripheral nuclear regions during the cell differentiation and maturation using computer-assisted density measurements at the single-cell level. The lineage of neutrophilic granulocytes in the bone marrow of patients suffering from chronic myeloid leukaemia was very convenient ...

In vitro studies of meiotic maturation of mouse oocytes have been carried out in the presence of several drugs. The individual steps of nuclear progression, including dissolution of the nuclear (germinal vesicle) membrane, condensation of dictyate chromatin into compact bivalents, formation of the first metaphase spindle, and extrusion of the first polar body, are each susceptible to one or mor...

Here we have used siRNAs and time-lapse epifluorescence microscopy to examine the roles of various candidate mitotic cyclins in chromatin condensation in HeLa cells. Knocking down cyclin A2 resulted in a substantial (∼7 h) delay in chromatin condensation and histone H3 phosphorylation, and expressing an siRNA-resistant form of cyclin A2 partially rescued chromatin condensation. There was no det...

We study a possible transition between symmetric nuclear matter and the diquark Bose-Einstein condensate (BEC) matter at zero temperature. We find that chiral restoration transition is first order and coincides with deconfinement. We investigate various possible coexistence patterns which may emerge from the first order deconfinement phase transition by assuming different values for the critica...

The critical density for kaon condensation in “nuclear star” matter is computed up to two-loop order in medium (corresponding to next-to-next-to-leading order in chiral perturbation theory in free space) with a heavy-baryon effective chiral Lagrangian whose parameters are determined from KN scattering and kaonic atom data. To the order considered, the kaon self-energy has highly non-linear dens...

1, 4-Diazabicyclo [2.2.2] octane (DABCO) was employed as a catalyst for one-pot synthesis of pyrano[2,3-d] pyrimidinone derivatives and MWNT- pyrimidinones from condensation reactions of aromatic aldehydes, malononitrile and thiobarbituric acid and MWNT-COCl . This method delivers the advantages of a simple method, environment friendly procedure, mild reaction conditions and high returns. The m...