Objective To develop a multiplex PCR protocol for routine screening of microdeletions on the Y chromosome Methods Five multiplex sets were established and Y chromosome microdeletions screening were carried out in 26 azoospermic men who undertook ICSI and 30 azoospermic men who undertook testicular biopsy. Results In 56 azoospermic men, 5 patients were found with AZFc/DAZ microdeletions, 2 patie...

BACKGROUND The causative mechanisms of male infertility are still poorly understood. Mutations in the Methylenetetrahydrofolate reductase (MTHFR) gene have been shown to be involved in male infertility; however, other mechanisms of pathogenesis, like promoter hyper-methylation, could also play a role. Therefore, in this study we compared the methylation status of the promoter region of MTHFR in...

BACKGROUND Different sperm retrieval procedures can help retrieve sperms that can be used for Intracytoplasmic Sperm Injection (ICSI) in azoospermic men. The objective was to compare the Intracytoplasmic sperm injection outcome, through different sperm retrieval procedures in azoospermic men. METHODS A Retrospective Study was carried out at Islamabad Clinic Serving Infertile Couples from Sept...

Background: About 15% of couples worldwide suffer from infertility problem that half of these cases are related to male infertility. Spermatogenesis is a cumulative process and thousands of genes are involved in it. Change in one of these genes or their products can cause male infertility. Tex11 is a germ cell specific gene that is located on the X chromosome (Xq13.1 region). This gene was iden...

OBJECTIVE To assess the effectiveness of the three-dimensional culture of spermatogenic cells in a collagen gel matrix from nonobstructive azoospermic patients and examine the relation between the success rate of in vitro spermatogenesis and serum FSH level as a diagnostic prediction. DESIGN Prospective study using radioimmunoassay, immunocytochemistry, and flow cytometry with primary culture...

BACKGROUND The use of ICSI has been a major breakthrough in the treatment of male infertility. Even azoospermic patients with focal spermatogenesis in the testis (not sufficient to spill over into the ejaculate) may benefit from the technique. Previous reports suggest a higher pregnancy rate after ICSI treatment in patients with obstructive azoospermia (OA) compared to their non-obstructive azo...

OBJECTIVE Analyze whether testicular histologic patterns from a group of azoospermic men with varicocele is predictive of treatment outcome after subinguinal microsurgical varicocele repair. MATERIALS AND METHODS Seventeen azoospermic men underwent bilateral open single testis biopsy and microsurgical subinguinal repair of clinical varicoceles. RESULTS Histopathology of testicular biopsies ...

Introduction. The etiology of male infertility characterized by non-obstructive azoospermia is largely unknown, especially at the molecular level. Identifying dysregulated microRNAs (miRNAs) in would be useful to achieve a more profound understanding its pathogenesis. Methods. Small RNA sequencing was performed on testicular tissues 10 nonobstructive azoospermic patients with Sertoli cell only ...