Produced by the Reactions with Bovine Hemo-globin Show Antimicrobial Activity Against Non-tuberculosis Mycobacteria. We studied the anti-microbial effects of phenoxazines produced by the reaction of o-aminophenol or its derivatives with bovine hemoglobin, on seven species of mycobacteria such as Mycobacterium tuberculosis , Mycobacterium marinum, Mycobacterium intracellulare, Mycobacterium scro...

the identification of mycobacteria in the species level has great medical importance. biochemical tests are laborious and time-consuming, so new techniques could be used to identify the species. this research aimed to the comparison of biochemical and sequencing 16s rdna gene methods to identify nontuberculous mycobacteria in patients suspected to tuberculosis in golestan province which is the ...

Three tuberculous, twenty-one non-tuberculous mycobacterial (NTM) reference strains and seventy two isolates classified by biochemical tests were shown to produce specific sets of DNA fragments in a polymerase chain reaction with single universal primer (UP-PCR). A rather wide limit of tolerance for variations in procedure of PCR mixture preparation and thermocycling parameters was found. There...

Although AIDS patients in other countries are frequently diagnosed as having atypical mycobacterial infection, in Southern Brazil there is a clinical impression that Mycobacterium tuberculosis is the rule rather than the exception. We made a retrospective review of cultures for mycobacteria at our hospital in order to determine the frequency of atypical mycobacteria and Mycobacterium tuberculos...

OBJECTIVE To determine and describe clinical symptoms, demographic characteristics and environmental exposures as determinants of pulmonary mycobacterial diseases among patients examined for tuberculosis in agropastoral communities in Northern Tanzania. METHODS This was a cross sectional study. Sputum samples were collected from patients attending three hospitals in Tanzania, and were investi...

BACKGROUND Human tuberculosis (TB), a chronic inflammatory disease is caused by Mycobacterium tuberculosis, a facultative intramacrophage pathogen. The highly complex interactions between mycobacteria and macrophages (MΦs), characterized in part by the induction and elaboration of several cytokines including IL-1, IL-6, IL-10, IL-12 p40 and IL-12 p70 are not yet fully understood. The cytokines ...

introduction: infections by multidrug resistant mycobacterium tuberculosis (mdr-tb) is a major public health challenge. secretion of proteins by m. tuberculosis plays an important role in the pathogenesis of the bacterium. we compared the protein profiles of susceptible m. tuberculosis and mdr-tb isolates using proteomic analyses, namely two dimensional gel electrophoresis (2de) and mass spectr...

OBJECTIVE To determine the incidence of Mycobacterium tuberculosis complex and non-tuberculous mycobacterial isolates in the routine setting of a large general hospital using an "in-house" multiplex polymerase chain reaction method and to establish a paradigm for the definitive identification of mycobacteria isolated using semi-automated equipment. METHODS Established tests, including polymer...