The present study demonstrates an efficient protocol for in vitro mass propagation of A. curassavica through direct as well as indirect regeneration. The effect of MS and L2 media supplemented with various concentrations and combinations of growth regulators has been studied. The growth regulators used include BAP and kinetin (cytokinins) and IAA, IBA, NAA and 2, 4-D (auxins). MS media proved b...

* Author for correspondence: Rozi Mohamed, Department of Forest Management, Faculty of Forestry, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Tel.: 60-3-8946 7183 (Office), Fax: 60-3-8943 2514. Email: rozimohd@putra. upm.edu.my. Abstract. A critical stage in the introduction of plants into tissue culture is to obtain cultures free from microbial contamination. This study in...

Abstract—Stevia rebaudiana Bertoni (natural sweetener) belongs to Asteraceae family and can be used as substitute of artificial sweeteners for diabetic patients. Conventionally, it is cultivated by seeds or stem cutting, but seed viability rate is poor. A protocol for callus induction and multiplication was developed to produce large no. of calli in short period. Surface sterilized nodal, leaf ...

A simple and efficient protocol for in vitro shoot and callus induction was established from three different explants of Canavalia gladiata (Jacq.) DC. an economically important legume. The explants viz., shoot buds, nodes, internodes were cultured in MS medium supplemented with various concentration and combination of (PGRs) Plant growth regulators 6-benzyl amino purine (BAP) (0.5mg/L – 1.5mg/...

Abstract. Adukonu IA, Ayeh KO, Acheampong E. 2022. Effective decontamination and multiplication of Croton membranaceus in vitro. Cell Biol Dev 6: 68-81. Müll. Arg. is a useful herb with medicinal properties, its leaves, roots, bark are used to treat diverse ailments. However, the harvesting method by traditional medical practitioners without replacement exposes it extinction. Therefore, only me...

Coffee plants can be micropropagated by nodal bud sprouting using the 6-benzylaminopurine (6BA) hormone. However, literature reports the use of a wide range of 6-BA, from 0.5 to 88.8 μM L-1. So, this study was performed to narrow that range. Nodal explants of Coffea arabica cv Mundo Novo obtained from in vitro plantlets were inoculated on gelled-MS medium supplemented with different concentrati...

A rapid in vitro propagation system has been established from mature shoot tip and nodal segments of ahighly valuable medicinal plant Alternanthera sessilis (L.). The explants were cultured on Murashige andSkoog’s medium augmented with different concentrations and combinations of plant growth regulators forshoot bud initiation and multiplications. For shoot tip, highest freque...

Micropropagation of Asclepias curassavica L. a medicinal plant valued for its medicinal properties has been carried out using two different plant culture media, Murashige and Skoog (MS) and L2. L2 medium proved to be superior to MS medium in terms of shoot multiplication and shoot length. Nodal explants showed enhanced organogenic response than shoot tip explants. Basal nodal explants produced ...

In vitro preservation of the “Kober 5BB” rootstock (Vitis berlandieri ˆ Vitis riparia) was assessed with the encapsulation technique and slow growth storage. Shoot tips and nodal segments excised from in vitro cultures were encapsulated in calcium-alginate beads. A 30 min ion exchange time proved optimal for forming proper beads. The encapsulated and naked explants were stored at 4 ̋C in the da...

An efficient and highly reproducible protocol for micropropagation of bird eye chilli Capsicum frutescens was attempted. Murashige and Skoog (MS) medium containing 0.5-3.0 mgl(-1) of 6-benzyladenine (BA), 2-isopentenyl adenine (2iP), kinetin and 0.5-2.0 mg l(-1) of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) along with 1 gl(-1) activated charcoal (AC) were used for shoot regeneratio...