BACKGROUND Oncogenic roles of epidermal growth factor receptor pathway substrate no.8 (EPS8) have been widely reported in various tumors, making targeting of EPS8 an appealing prospect. Here, we describe the role of EPS8 in acute myeloid leukemia (AML) and consider the potential of EPS8 as an anti-AML target. Nuclear localization signal (NLS) residues of tumor-associated proteins are crucial fo...

Acute promyelocytic leukemia (APL) is characterized by the presence of the promyelocytic leukemia (PML)-retinoic acid receptor-α (RAR-α) fusion protein. PML-RARα can be cleaved by neutrophil elastase (NE) in several positions in cells in the promyelocytic stage, nuclear location signal (NLS)-negative PML and NLS-RARα may be the products of PML-RARα by NE. The function of NLS-RARα may be affecte...

The development of an efficient delivery system is critical for the successful treatment of cardiovascular diseases using non‑viral gene therapies. Cytoplasmic and nuclear membrane barriers reduce delivery efficiency by impeding the transfection of foreign genes. Thus, a gene delivery system capable of transporting exogenous genes may improve gene therapy. The present study used a novel strateg...

Alterations in protein Subcellular localization often contribute to the development of human diseases. Post-transcriptional modifications, such as phosphorylation on the Nuclear Localization Signal (NLS), may change the protein’s localization. However, little is known about the frequency and local effects of phosphorylation near NLS sites. In this study, a computer program was developed to sear...

The transport of proteins into the nucleus is a receptor-mediated process that is likely to involve between 50-100 gene products, including many that comprise the nuclear pore complex. We have developed an assay in Saccharomyces cerevisiae for the nuclear transport of green fluorescent protein fused to the SV-40 large T antigen nuclear localization signal (NLS-GFP). This assay allows the measur...

We show that discretization effects in finite-difference simulations of blowup solutions of the nonlinear Schrödinger equation (NLS) initially accelerate self focusing but later arrest the collapse, resulting instead in focusing–defocusing oscillations. The modified equation of the semi-discrete NLS, which is the NLS with highorder anisotropic dispersion, captures the arrest of collapse but not...

The mechanism by which phosphorylation regulates nuclear localization sequence (NLS)-dependent nuclear protein import is largely unclear. Whereas nuclear accumulation of SV40 large tumor antigen (T-ag) fusion proteins is completely dependent on the T-ag NLS (amino acids 126-132), the rate of nuclear import is increased 50-fold by amino acid residues 111-125 and in particular a site for the prot...

The multifunctional nuclear inclusion protein a (NIa) of potyviruses (genus Potyvirus; Potyviridae) accumulates in the nucleus of virus-infected cells for unknown reasons. In this study, two regions in the viral genome-linked protein (VPg) domain of NIa in Potato virus A (PVA) were found to constitute nuclear and nucleolar localization signals (NLS) in plant cells (Nicotiana spp). Amino acid su...

Using the inverse spectral theory of the nonlinear Schrödinger (NLS) equation we correlate the development of rogue waves in oceanic sea states characterized by the JONSWAP spectrum with the proximity to homoclinic solutions of the NLS equation. We find in numerical simulations of the NLS equation that rogue waves develop for JONSWAP initial data that is “near” NLS homoclinic data, while rogue ...

Dodecyl protocatechuate (dodecyl) is a derivative of protocatechuic acid (3,4-dihydroxybenzoic acid) that possesses anti-oxidant and antifungal properties. Nanostructured lipid systems (NLS) can potentiate the action of many antifungal agents, reducing the required dose and side effects by improving their activity. This work aimed to evaluate dodecyl protocatechuate loaded into a NLS (NLS+dodec...