AN account of the catalytic destruction of carnosine in vitro at 1000 by a substance present in skeletal muscle and liver has been previously published [Clifford, 1922]. These experiments showed a progressive loss of the iminazole ring when a solution containing carnosine was kept with skeletal muscle or liver over a period of 3-4 weeks. The amount of iminazole was estimated by a colorimetric m...

Here we describe a fluctuation-based method to quantify how protein oligomerisation modulates signalling activity of a multifunctional protein. By recording fluorescence lifetime imaging microscopy (FLIM) data of a FRET biosensor in a format that enables concomitant phasor and cross Number and Brightness (cN&B) analysis, we measure the nuclear dynamics of a Rac1 FRET biosensor and assess how Ra...

Two or possibly three equivalents of N-bromoacetylacetazolamide interact with two histidine residues located in the active site region of human carbonic anhydrase B. The most rapid reaction occurs at the l-nitrogen of a histidine residue and this is followed by a slower alkylation of the 3-nitrogen of the same histidine and the 3-nitrogen of a second histidine. From a reaction mixture of N-brom...

Increasing concern over the air pollution has forced the man to develop the new technology for the better quality of life. In modern world, air pollution does not need any introduction. One of the major factors responsible for such degradation in the quality of life is air pollution. However, chief among them are carbon monoxide, sulphur dioxide, nitrogen oxides, ozone, etc. Nitrogen dioxide ca...

The utility of allylsamarium bromide, both as a nucleophilic reagent and a single-electron transfer reagent, in the reaction of carbonyl compounds with allylsamarium bromide in the presence of diethyl phosphate is reported in this communication. From a synthetic point of view, a simple one-pot method for the preparation of terminal olefins is developed.

Unlike its other halogen atom siblings, the utility of chlorinated arenes and (hetero)arenes are twofold: they are useful in tuning electronic structure as well as acting as points for diversification via cross-coupling. Herein we report the invention of a new guanidine-based chlorinating reagent, CBMG or "Palau'chlor", inspired by a key chlorospirocyclization en route to pyrrole imidazole alka...

Spermidine, spermine and other polyamines 1—5 were selectively protected at the terminal primary amino functions without affecting the secondary amino groups using N-ethoxycarbonylphthalimide (15), the Nefkens’ reagent. Three representative products, 17, 18 and 20, readily underwent acylation at the secondary amino nitrogen to give the corresponding compounds 21—26. Selective deprotection of tw...

A new reagent, fluorescamine, provides a simple, rapid fluorometric measurement of primary amino compounds in seawater. The reagent detects as little as 0.2 r.lM (glycine equivalents) directly in 2 ml of seawater. Fluorescamine-positive primary amines in the region of Newport Bay, California, range from about 0.3 PM in nearshore waters to 14 PM in bay sediments. About half the fluorescamine-pos...

The traceless Staudinger ligation is an effective means to synthesize an amide bond between two groups of otherwise orthogonal reactivity: a phosphinothioester and an azide. An important application of the Staudinger ligation is in the ligation of peptides at a variety of residues. Here, we demonstrate that the traceless Staudinger ligation can be achieved in water with a water-soluble reagent....

A direct method is described, in which a single reagent is used without deproteinization, for measuring blood urea nitrogen (BUN) in serum. The reagent is a mixture of diacetylmonoxime, antipyrine (phenazone), and arsenic pentoxide in acid solution. Lipemia, bilirubin to 25 mg/100 ml, hemoglobin to 1g/100 ml, and protein to 20g/100 ml do not affect the results. Beer’s law is followed at 460 nm ...