The effect of formyl chemotactic peptide (fCTP, fMet-Leu-Phe), beta-amyloid peptides (beta-AP, 1-42, 1-16 and 25-35), and bradykinin (BK) on functional activity of phagocytic cells has been investigated. Wheat germ agglutinin (WGA) was also used as a model membrane binding agent of polypeptide nature. Murine monocyte-macrophage cell line J774.2 and normal human blood polymorphonuclear (PMN) cel...

To explain anaerobic nitrite/nitrate production at the expense of ammonium mediated by manganese oxide (Mn(IV)) in sediment, nitrate and manganese respirations were investigated in a strain (Shewanella algae C6G3) presenting these features. In contrast to S. oneidensis MR-1, a biotic transitory nitrite accumulation at the expense of ammonium was observed in S. algae during anaerobic growth with...

The feasibility of removing nitrogen from mature landfill leachate using the nitrite route was investigated in a two-stage SBR system – (1 first stage, short-cut nitrification; second stage, denitrification via nitrite. With a volumetric exchange rate (n) of 0.3 d and an oxygen concentration of between 1.2 and 1.3 mg/L, the nitrite accumulation ratio – (NO2-N)×100/(NOx-N)% – was between 32% and...

Nitrite is an intermediate in the oxidation of ammonium to nitrate. An elevated ambient nitrite concentration is a potential problem for freshwater fish since nitrite is actively taken up across the gills in competition with chloride. Nitrite is a well-known toxicant for fish as well as a disrupter of multiple physiological functions including ion regulatory, respiratory, cardiovascular, endocr...

The purpose of this study was to investigate the effects of aspirin-like drugs on nitric oxide (NO) synthesis in rat vascular smooth muscle cells (VSMCs). We measured the accumulation of nitrite, a stable oxidation product of NO, and the expression of inducible NO synthase (iNOS) mRNA and protein in rat cultured VSMCs. Sodium salicylate, aspirin, and indomethacin dose-dependently enhanced nitri...

Heterotrophic bacteria were studied which produced nitrite in the presence of ammonia and in the absence of nitrate. A soil extract medium was prepared which allowed good growth as well as nitrite production in the absence of nitrate. Quantitative data are recorded showing that ammonia decreases as nitrite accumulates when four different cultures are grown in the soil medium. Resting cell studi...

The course of denitrification of nitrate in static cultures of Paracoccus denitrificans was studied. Reduction of nitrate to gaseous nitrogen without accumulation of nitrite because of parallel and balanced activities of nitrate and nitrite reductases was observed in nutrient broth. In minimal liquid cultures supplemented with either methanol, acetate, or ethanol as a sole carbon source, substa...

The purpose of this study was to investigate the effects of aspirin-like drugs on nitric oxide (NO) synthesis in rat vascular smooth muscle cells (VSMCs). We measured the accumulation of nitrite, a stable oxidation product of NO, and the expression of inducible NO synthase (iNOS) mRNA and protein in rat cultured VSMCs. Sodium salicylate, aspirin, and indomethacin dose-dependently enhanced nitri...