Serum samples from 315 horses from Costa Rica, Central America, were examined for the presence of antibodies against Sarcocystis neurona, Neospora spp., and Toxoplasma gondii by using the surface antigen (SAG) SnSAG2 enzyme-linked immunosorbent assay (ELISA), the NhSAG1 ELISA, and the modified agglutination test, respectively. Anti- S. neurona antibodies were found in 42.2% of the horses by usi...

Neosporosis is an important cause of bovine abortion worldwide. Many aspects of transmission of Neospora caninum in nature are unknown. The white-tailed deer (Odocoileus virginianus) is considered one of the most important wildlife reservoirs of N. caninum in the USA. During the hunting seasons of 2008, 2009, and 2010, brains of 155 white-tailed deer fetuses were bioassayed in mice for protozoa...

Deer are considered important intermediate hosts for the coccidian parasites, Toxoplasma gondii and Neospora caninum. Antibodies to N. caninum and T. gondii were determined in sera of 42 mule deer (Odocoileus hemionus hemionus) and 43 black-tailed deer (Odocoileus hemionus columbianus) from Washington state, USA, using direct agglutination test with specific antigens. A titer of 1:25 was consid...

The purpose of the present study was to investigate the potential of Neospora caninum oocysts to infect sheep and determine whether N. caninum DNA could be detected by polymerase chain reaction (PCR) assay in blood and brain of sheep after oocyst inoculation. Six ewes were inoculated per os with 10(4) N. caninum oocysts, whereas 2 ewes served as uninoculated controls. All sheep were bled weekly...

visceral leishmaniasis (vl) is one of the most important parasitic diseases which is endemic in different parts of iran. serological studies were conducted by direct agglutination test (dat) on 12144 human serum samples, collected from four geographical zones of iran. sero prevalence, geographical distribution, clinical signs and symptoms for human visceral leishmaniasis based on dat for the pe...

Parasite-specific antibody responses were detected using an indirect fluorescent antibody (IFA) test in cattle that were naturally or experimentally infected with Neospora parasites. The test was developed using Neospora tachyzoites isolated from an aborted bovine fetus and grown in bovine cell cultures (isolate BPA1). In all cases, infections were confirmed by the identification of Neospora ta...

BACKGROUND Neospora caninum is a protozoan parasite from phylum apicomplexa and an important agent causing abortion in cattle which produce notable economic loss all around the world. METHODS Dot-Elisa was set up performing checker board procedure and then 178 sera of cattle examined with commercial indirect ELISA and direct agglutination test (DAT) were also evaluated by dot-ELISA afterwards...

background: toxoplasma gondii infection is widely prevalent in many species of warm-blooded animals including human. the aim of this study was to determine the prevalence of antibodies to t. gondii from slaughtered sleep and goats by mod­ified agglutination test (mat) in kerman region , southeastern iran. methods : altogether 1340 blood samples were collected from 562 sheep and 778 goats from a...

background: until now, the comparison of the rk39 strip test (rkt) and direct ag-glutination test (dat) for detection of visceral leishmaniasis (vl) is exclusively based on either positive or negative qualification of the reaction outcome. objective: in this study, we compared the diagnostic performance of rkt and dat for vl both qualitatively and semi-quantitatively. methods: for comparison ba...