the armadillo could be considered as an experimental model for leprosy studies. mycobacterium leprae main host cells are macrophages and schwann cells. however, endocytosis and germicidal activities of armadillo macrophages and monocytes have not yet been evaluated. the aim of this research was to evaluate endocytosis and nbt-reduction activities of monocyte-derived macrophages (mdm) and monocy...

Jan Samuelsson, MD, PhD, Department of Medicine, Section for Hematology, Karolinska Institute at Stockholm Söder Hospital, S-118 83 Stockholm (Sweden) We read with interest the report by Piva et al. [1] describing an impaired function of the NADPH oxidase, assessed as the reduction of nitroblue tetrazolium (NBT) after stimulation with phorbol myristate acetate (PMA), in patients with various st...

The purpose of this study was to evaluate the ability of spermatozoa and leukocytes in semen to produce reactive oxygen species (ROS) by using nitroblue tetrazolium (NBT) staining and to examine the association between NBT staining and levels of ROS as measured by chemiluminescence. Twenty-one infertility patients (leukocytospermia; n = 8; nonleukocytospermia, n = 13) and 9 healthy donors were ...

The ability of highly purified human leukocytic pyrogen (LP) to stimulate neutrophil oxygen-dependent metabolism was studied. Human peripheral blood neutrophils exposed to leukocytic pyrogen in vitro demonstrated an increase in the percentage of neutrophils reducing nitroblue tetrazolium (NBT) dye and a marked stimulation of superoxide dismutase inhibitable reduction of ferricytochrome c. LP st...

Normal human granulocytes are able to reduce the yellow dye, nitroblue tetrazolium (NBT), to insoluble blue formazan. This has been a useful reaction for the recognition of chronic granulomatous disease of childhood (CGD) and the carrier state, because granulocytes froin these patients are deficient in their ability to reduce this dye(l). It has also been suggested that granulocytes from patien...

Human T cell lymphotropic Virus type-1 (HTLV-1) induces lymphocyte activation and proliferation, but little is known about the innate immune response due to HTLV-1 infection. We evaluated the percentage of neutrophils that metabolize Nitroblue tetrazolium (NBT) to formazan in HTLV-1 infected subjects and the association between neutrophil activation and IFN-gamma and TNF-alpha levels. Blood was...

Phagocytes from X-linked chronic granulomatous disease (X-CGD) patients are deficient in their ability to generate superoxide because of a defective gene that encodes a heavy chain of cytochrome b, a critical component in the superoxide-generating pathway. Previously we have shown that a single in vivo treatment of selected X-CGD patients with interferon-gamma (INF-gamma) resulted 14 days later...

Quantitative and histochemical nitroblue with polycythemia vera and neoplasia astetrazolium reduction (NBT) tests were persociated with fever, as well as in those with formed on leukocytes of 13 patients with known bacterial infection. It is suggested polycythemia vera, seven with chronic that patients with neoplasia or polycygranulocytic leukemia, eight with neothemia vera may show a false pos...