To examine the possibility that NaF enhances phosphoinositide-specific phospholipase C (PIC) activity in neural tissues by a mechanism independent of a guanine nucleotide binding protein (Gp), we have evaluated the contribution of Gp activation to NaF-stimulated phosphoinositide hydrolysis in human SK-N-SH neuroblastoma cells. Addition of NaF to intact cells resulted in an increase in the relea...

The effects of simulated orthodontic forces such as centrifugal force or water pressure on sodium fluoride (NaF)-induced cytotoxicity against mouse osteoblast-like cells MC3T3-E1 were investigated. Loading with centrifugal force (44.5 g/cm2) or water pressure (5 g/cm2) slightly reduced the cell proliferation and additively enhanced the cytotoxic activity of millimolar concentrations of NaF. NaF...

It was hypothesized that NaF induces calcium sensitization in Ca(2+)-controlled solution in permeabilized rat mesenteric arteries. Rat mesenteric arteries were permeabilized with beta-escin and subjected to tension measurement. NaF potentiated the concentration-response curves to Ca(2+) (decreased EC(50) and increased E(max)). Cumulative addition of NaF (4.0, 8.0 and 16 mM) also increased vascu...

Nipple aspirate fluid (NAF) requires investigation as a potential source of biomarkers for early diagnosis or risk assessment in breast cancer and other breast disorders. The present study demonstrated that proteins were easily extracted from dried NAF spots on Guthrie cards and were suitable for mass spectrometry analysis. NAF was obtained from 80 women, collected on Guthrie cards, between 200...

BACKGROUND Concentrations of estrogen and progesterone within the breast could provide a better reflection of breast cancer risk than levels in the circulation. We developed highly sensitive immunoassays for multiple steroid hormones and proteins in the nipple aspirate fluid (NAF), which can be obtained noninvasively with a simple suction device. Previous studies showed that NAF hormone levels ...

BACKGROUND Cyclooxygenase enzymes (COX-1, COX-2, and COX-3) convert arachidonic acid to prostaglandins, prostacyclins, thromboxanes, and other hydroxy fatty acids. Among these, prostaglandin E(2) (PGE(2)) has tumor growth-promoting activity. The COX-2 isoform is the primary enzyme involved in PGE(2) production in cancerous tissue. OBJECTIVE/HYPOTHESIS We administered the COX-2 inhibitor celec...

In this paper, we incorporate the co-Z arithmetic with Protected NAF to build a dual-field scalar multiplication method in ECC (elliptic curve cryptography) with lower time complexity and higher security, called co-Z Protected NAF. The Protected NAF is the alteration of the original NFA against SPA (simple power analysis) attack. But the employed dummy operations, double-and-add-always, often r...

The biological properties of a neutrophil-activating factor (NAF), which was recently identified as a novel peptide of approximately 6,000 mol wt, are described. NAF is produced de novo by human blood monocytes upon stimulation with LPS, PHA, and Con A. It induces two main responses in human neutrophils, i.e., exocytosis (release from specific granules in normal, and from specific and azurophil...

The NH2 terminus of the F1 subunit of the paramyxovirus SV5 fusion protein (fusion related external domain; FRED) is a hydrophobic domain that is implicated as being involved in mediating membrane fusion. We have examined the ability of the FRED to function as a combined signal/anchor domain by substituting it for the natural NH2-terminal signal/anchor domain of a model type II integral membran...