Abbreviations: CAPS: N-cyclohexyl-3-aminopropanesulfonic acid cDNA: omplementary DNA to mRNA CHES: N-cyclohexyl-2-aminoethanesulfonic acid. DTT: dithiothreitol FPLC: fast protein liquid chromatography IPTG: isopropylthio-β-D-galactoside LB: Luria-Bertani MES: 2-(N-morpholino)ethanesulfonic acid Ni-NTA: Nickel-nitriloacetic acid ORF: open reading frame PCR: polymerase chain reaction PMSF: phenyl...

Non-peptidyl postatin analogues, (S)-N-substituted-2-[2-(1-acylpyrrolidinyl)]-2-oxoacetamides were synthesized and examined for their inhibitory activity against prolyl endopeptidase and cathepsin B in vitro. Many compounds showed stronger activity than natural poststatin, a pentapeptide. Among them, (S)-N-cyclohexyl-2-oxo-2-[2-(1-(3-phenoxybenzoyl)pyrrolidinyl)]ace tamide (22) and (S)-N-cycloh...

cui nanoparticles as an expedient and recyclable catalyst for the synthesis of n-cyclohexyl-3-aryl-quinoxaline-2-amines in ethanol via a multi-component reaction are established. the products were separated from the catalyst simply by filtration. the catalyst could be recycled and reused for several times without noticeably decreasing the catalytic activity.