Biocatalytic processes often encounter problems due to toxic reactants and products, which reduce biocatalyst viability. Thus, robust organisms capable of tolerating or adapting towards such compounds are of high importance. This study systematically investigated the physiological response of Pseudomonas taiwanensis VLB120∆C biofilms when exposed to n-butanol, one of the potential next generati...

BACKGROUND n-Butanol and isobutanol produced from biomass-derived sugars are promising renewable transport fuels and solvents. Saccharomyces cerevisiae has been engineered for butanol production, but its high butanol sensitivity poses an upper limit to product titers that can be reached by further pathway engineering. A better understanding of the molecular basis of butanol stress and tolerance...

The whole plant, Pergularia daemia (Family: Asclepediaceae), was extracted with 50% alcohol and a fresh batch of the plant material was successively extracted with petroleum ether, ethyl acetate and n-butanol to determine its diuretic activity. The diuretic activity of the different extracts at a dose of 400 mg/Kg was assessed orally in rats with furosemide as a standard drug using Lipschitzs t...

n-Butanol has several favourable properties as an advanced fuel or a platform chemical. Bio-based production of n-butanol is becoming increasingly important for sustainable chemical industry. Synthesis of n-butanol can be achieved via more than one metabolic pathway. Here we report the metabolic engineering of Saccharomyces cerevisiae to produce n-butanol through a synergistic pathway: the endo...

Immobilized Candida antarctica lipase B, Novozym 435, was used as the biocatalyst in the esterification of adipic acid with four different isomers of butanol (n-butanol, sec-butanol, iso-butanol, and tert-butanol). Optimum conditions for the synthesis of adipate esters were obtained using response surface methodology approach with a four-factor-five-level central composite design concerning imp...

The whole plant, Pergularia daemia (Family: Asclepediaceae), was extracted with 50% alcohol and a fresh batch of the plant material was successively extracted with petroleum ether, ethyl acetate and n-butanol to determine its diuretic activity. The diuretic activity of the different extracts at a dose of 400 mg/Kg was assessed orally in rats with furosemide as a standard drug using Lipschitzs t...

Oligodeoxynucleotides are among the most useful tools in modem molecular biology. Phosphoramidite-synthesized oligonucleotides require a final reaction step with concentrated ammonia to effect their complete deprotection. The resulting organic by-products, e.g. benzamides, need to be removed from the oligonucleotides before the latter may be used in various enzymatic processes (1). Typically, t...

The article presents the results of research on impact feeding marine reciprocating internal combustion engines with blends diesel fuel and n-butanol their performance parameters. study includes a plan empirical results, in which engine efficiency emissions harmful compounds exhaust gases were determined. indicates that it is resonable to use containing n-butanol. N-butanol positively affect co...

A reduced primary reference fuel (PRF)-alcohol-di-tert-butyl peroxide (DTBP) mechanism with 108 species and 435 reactions, including sub-mechanisms of PRF, methanol, ethanol, DTBP, and the four butanol isomers, is proposed for homogeneous charge compression ignition (HCCI) engine combustion simulations of butanol isomers/nheptane mixtures. HCCI experiments fueled with butanol isomer/n-heptane m...

background insomnia or sleeplessness is a disorder characterized by a personal incapability to falling or staying asleep for a desirable period of time, therefore disturb in quality of life. objectives the present study was carried out to investigate the effect of hydro-alcoholic extract of nepeta glomerulosa and its fractions on sleep-prolonging. materials and methods the extract and its fract...