Contagious caprine pleuropneumonia (CCPP) is caused by Mycoplasma capricolum subsp. capripneumoniae (Mccp) which belongs to the Mycoplasma mycoides cluster, a group of five closely related Mycoplasmas, pathogenic to ruminants. The true lesions of CCPP are restricted to the alveolar tissues of infected goats, which distinguish it from other respiratory diseases of small ruminants caused by membe...

DNA amplification techniques offer considerable promise for the identification of Mycoplasma mycoides cluster members. They avoid antigenic cross-reactivity and variability that hamper serological methods. Many sets of primers, specific of these different members and of Mycoplasma putrefaciens, have been proposed. To assess the reliability of some of these PCR tests in routine laboratory diagno...

Different physical factors like aeration, temperature and shaking culture conditions were applied to the culture and observation of growth was studied for 72 hours of incubation. Similarly the Mycoplasma mycoides subspecies capri was grown in PPLO medium enriched with different concentrations (5, 10 and 20%) of serum from horse, bovine and allantoic fluid, along with usual concentration of yeas...

Rapid and specific detection of Mycoplasma mycoides subsp. mycoides Small Colony (M. mycoides SC) is important for the effective control of contagious bovine pleuropneumonia. Although the United States has been free of this disease for over 100 years, it is necessary to develop modern diagnostic assays that are sensitive and specific for biological agents that would affect the US agricultural i...

  I n a survey of pneumonia due to Mycoplasma 282 out of 12168 ovine and caprine lung condemnation were collected (2.32%). Mycoplasma spp. has been isolated from pneumonic cases in 4 sheep and 2 goats. PCR studies were confirmed the genus of Mycoplasma although attempting for identification of strains M. mycoides, M. capricolum/caprine pleuropneumonia and M. arginin were in failure. The lesions...

Infections caused by Mycoplasma species belonging to the 'mycoides cluster' negatively affect the agricultural sector through losses in livestock productivity. These Mycoplasma strains are resistant to many conventional antibiotics due to the total lack of cell wall. Therefore, there is an urgent need to develop new antimicrobial agents from alternative sources such as medicinal plants to curb ...

A gene probe, CAP-21, which demonstrated interrelationships between the members of the Mycoplusma mycoides cluster w a s developed. The probe easily differentiated mycoplasmas in this cluster by clear and predictable hybridization patterns in Southern blots and separated the cluster into four groups. Strains of M. mycoides subsp, mycoides which were capable of causing contagious bovine pleuropn...

Contour clamped homogeneous field (CHEF) agarose gel electrophoresis (AGE), ramped to give linear separation of DNA molecules of 600-1600 kilobase pairs (kbp), was used to determine mobilities for full-sized genomic DNA of the serotype standard strains of the human genital mollicutes, Ureaplasma urealyticum relative to yeast chromosomal DNA markers. Indicated genome sizes (in kbp) were 760 for ...

In vitro minimum inhibitory concentrations were determined for 21 antimicrobials against 41 isolates of Mycoplasma mycoides subsp. mycoides small-colony type, the cause of contagious bovine pleuropneumonia. Of the antimicrobials used most widely in Africa, oxytetracycline and tilmicosin were effective, while the isolates were resistant to tylosin. These results provide a baseline for monitoring...

The phylogenetic position of the Mollicutes has been re-examined by using phosphoglycerate kinase (Pgk) amino acid sequences. Hitherto unpublished sequences from Mycoplasma mycoides subsp. mycoides, Mycoplasma hyopneumoniae and Spiroplasma citri were included in the analysis. Phylogenetic trees based on Pgk data indicated a monophyletic origin for the Mollicutes within the Firmicutes, whereas B...