The aim of the present study was to detect and identify Mycobaterium spp. in 50 samples coalho cheese sold Northeast region Brazil. Of analyzed samples, 35 were produced by artisanal process, using raw milk, 15 originated from industrialized establishments that pasteurize milk. Conventional real-time nested PCR for rv2807 gene M. tuberculosis complex performed directly samples. Samples grown si...

it is difficult to distinguish between clinically significant slowly-growing, non-pigmented mycobacteria, notably to separate m. aviumand m. intracellulare from one another and from m. scrofulaceum strains. the purpose of this study was to evaluate the extent to which 16s rrna sequencing could be used to highlight the taxonomic relationships of the mycobacterial strains, which are difficult to ...

It is difficult to distinguish between clinically significant slowly-growing, non-pigmented mycobacteria, notably to separate M. aviumand M. intracellulare from one another and from M. scrofulaceum strains. The purpose of this study was to evaluate the extent to which 16S rRNA sequencing could be used to highlight the taxonomic relationships of the mycobacterial strains, which are difficult to ...

Pulmonary tuberculosis, is a granulomatous disease of the lungs that caused by Mycobaterium tuberculosis. A number of genes have been identified in studies of diverse origins to be important in tuberculosis. Out of which the tumor necrosis factor α (TNF-α) gene has been identified to plays a major role in the initial and long-term control of tuberculosis. In this study, the TNF-α gene at -238, ...

The putative proteasome-associated proteins Mpa (Mycobaterium proteasomal ATPase) and PafA (proteasome accessory factor A) of the human pathogen Mycobacterium tuberculosis (Mtb) are essential for virulence and resistance to nitric oxide. However, a direct link between the proteasome protease and Mpa or PafA has never been demonstrated. Furthermore, protein degradation by bacterial proteasomes i...

The main objective of this study was to detect association between microsatellite genetic markers and a candidate gene with tuberculosis-related traits in African zebu cattle. A total of 249 of Chadian zebu cattle was genotyped for 23 microsatellites and for a known candidate gene NRAMP1 (natural resistance associated macrophage protein 1). These animals were measured for two tuberculosis-relat...

BACKGROUND Tuberculosis is a disease with high morbidity, caused mainly by Mycobaterium tuberculosis (M.tb.). DNA vaccines show a promising future due to their unique advantages over conventional methods. The early-secreted antigen target (ESAT)-6 and culture filtrate protein (CFP)-10 of M.tb. antigens have been identified as vaccine candidates against Mycobacteria and used as subunit vaccines,...