HIGHLIGHTS A multiplex PCR to identifying mycobacteria of medical interest was evaluated. Mycobacterium tuberculosis can be differentiated from other with the assay. The assay showed high sensitivity and specificity in detecting M. avium complex.

extrapulmonary tuberculosis is a complication of pulmonary tuberculosis that is disseminated through hematogenous or lymphatic system and may involve any organs. we present a case of primary tuberculosis of the penis that is a very rare presentation of tuberculosis. patient the patient was a 43-year-old man who had kidney transplantation. he admitted with painful ulceration on the glans of peni...

conclusions: pcr method is a valuable, cost-effective and alternative tool for quick diagnosis of active tuberculosis in different clinical specimens. results: the concordance rate between the three sets of primers was calculated and is6110/buffer pcr method showed good agreement with the lj culture method (κ = 0.627, p < 0.0001). the sensitivity of is6110/buffer pcr was 58.33%, with specificit...

Tuberculosis is a worldwide contagious and chronic disease of human as well as domestic animals with zoonotic potential. The Mycobacterium bovis and Mycobacterium tuberculosis are the main cause of tuberculosis. It has worldwide distribution with significant effect on animals and has public health importance. Therefore the present study was conducted to determine the prevalence of Mycobacterium...

A PCR specific for spacer regions 33 and 34 of the direct repeat region of the Mycobacterium tuberculosis complex was developed to complement the biochemical differentiation of M. tuberculosis, Mycobacterium bovis, M. bovis BCG, and Mycobacterium africanum subtypes I and II. In addition, this approach was incorporated into a multiplex PCR that included primers specific for IS6110 and the 65-kDa...

Background: Paratuberculosis and tuberculosis (TB) caused by Mycobacterium avium paratuberculosis (MAP) and Mycobacterium tuberculosis complex (MTC), respectively are economically important, chronic debilitating diseases affecting the dairy herds and are also potential zoonotic threats. Aims: Differential diagnosis of paratuberculosis and TB i...

STUDY OBJECTIVES The detection of Mycobacterium sp on a culture remains the "gold standard" technique for the diagnosis of mycobacterial infections. A small percentage of these cultures, however, may be contaminated by other nonfastidious microorganisms, making accurate diagnosis difficult. We evaluated the use of a polymerase chain reaction (PCR) protocol that was specific for the genus Mycoba...

abstract background and objective: a high level of homogeneity observed within all bacteria in the mycobacterium tuberculosis complex makes a property that seriously challenges traditional biochemical-based identification methods of these pathogens in the laboratory. the work presented here was conducted to characterize mycobacterium tuberculosis complex isolates in golestan, northern iran. mat...

objective(s) isoniazid (inh) is one of the main first line drugs used in treatment of tuberculosis and development of resistance against this compound can result in serious problems in treatment procedures. resistance to inh is mediated mainly by mutation in katg gene that is coded for the catalase enzyme. the proportional method for detection of inh-resistance is time consuming due to the slow...

Background. Tuberculosis is one of the world's health problems, with larger mortality rate than HIV/AIDS. In order to control this disease, a new and inexpensive diagnostic method needed. Oral samples, such as buccal mucosa epithelium, can be an alternative specimen, other sputum, in diagnosis tuberculosis. Purpose. This study aimed analyze presence Mycobacterium tuberculosis on swabbing patien...