INTRODUCTION The objective of heritable mutagenesis studies is to evaluate some toxic chemical’s potential to induce genetic damage that leads to disorders in offspring of test subjects, i.e., transmissible genetic damage induced in the subjects’ germ cells (Maxwell and Newell, 1973; Russell and Kelly, 1982; Shelby, 1988; Shelby, et al., 1993). Typically, laboratory animals are employed as test...

Saturation mutagenesis is a powerful technique for engineering proteins, metabolic pathways and genomes. In spite of its numerous applications, creating high-quality saturation mutagenesis libraries remains a challenge, as various experimental parameters influence in a complex manner the resulting diversity. We explore from the economical perspective various aspects of saturation mutagenesis li...

The hammerhead structural model proposed for sequences that mediate self-cleavage of certain RNAs contains base-paired three stems and 13 conserved bases. Insertion, deletion and base substitution mutations were carried out on a 58 base RNA containing the sequence of the single-hammerhead structure of the plus RNA of the virusoid of lucerne transient streak virus, and the effects on self-cleava...

human granulocyte colony stimulating factor (hg-csf) induces proliferation and differentiation of granulocyte progenitor cells. this glycoprotein is currently being used for treatment of neutropenia, in patients who have undergone bone marrow transplantation. so far, different researchers have tried to enhance hg-csf biological activity and stability. in this study, polymerase chain reaction (p...

word count: 178 Manuscript word count: 4936 Blood First Edition Paper, prepublished online June 16, 2005; DOI 10.1182/blood-2004-12-4840 Copyright © 2005 American Society of Hematology only. For personal use at PENN STATE UNIVERSITY on February 23, 2013. bloodjournal.hematologylibrary.org From