As an important industrial enzyme, protease is widely used in feed, food and other fields. At present, the insufficient activity obtained from microorganisms cannot meet purpose of production. In this study, Bacillus amyloliquefaciens with high production was screened animal feces by plate transparent circle method. To improve protease, atmospheric room temperature plasma (ARTP) mutagenesis fir...

The current SARS-CoV-2 pandemic underscores the importance of understanding evolution RNA genomes. While is subject to formation similar lesions as DNA, evolutionary and physiological impacts have on viral genomes are yet be characterized. Lesions that may drive can induce breaks repaired by recombination or cause base substitution mutagenesis, also known editing. Over past decade so, editing m...

It has been proposed that spin-polarized cosmic radiation can induce asymmetric changes in helical biopolymers may account for the emergence of biological homochirality. The parity violation weak interaction direct consequences on transport polarization ray showers. In this paper, we show muons retain their down to energies at which they initiate enantioselective mutagenesis. Therefore, are mos...

The OB-fold is a diverse structure superfamily based on a beta-barrel motif that is often supplemented with additional non-conserved secondary structures. Previous deletion mutagenesis and NMR hydrogen exchange studies of three OB-fold proteins showed that the structural stabilities of sites within the conserved beta-barrels were larger than sites in non-conserved segments. In this work we exam...

macrophomina phaseolina is one of the major yield limiting factors of melons in tropical and subtropical regions. for eco-friendly and effective management of the disease, 24 gamma induced mutants from trichoderma harzianum were evaluated against three isolates of the pathogen representing three geographically different regions viz. khorasan (isolate 1), garmsar (isolate 2) and khuzestan (isola...

A major challenge in the post-genome era of plant biology is to determine the functions of all the genes in the plant genome. A straightforward approach to this problem is to reduce or knock out expression of a gene with the hope of seeing a phenotype that is suggestive of its function. Insertional mutagenesis is a useful tool for this type of study, but it is limited by gene redundancy, lethal...